Drummond Revel S M, Lee Hui Wen, Luo Zhiwei, Dakin Jack F, Janssen Bart J, Snowden Kimberley C
The New Zealand Institute for Plant and Food Research Limited, Auckland, New Zealand.
Front Plant Sci. 2023 Oct 11;14:1277617. doi: 10.3389/fpls.2023.1277617. eCollection 2023.
The action of the petunia strigolactone (SL) hormone receptor DAD2 is dependent not only on its interaction with the PhMAX2A and PhD53A proteins, but also on its expression patterns within the plant. Previously, in a yeast-2-hybrid system, we showed that a series of a single and double amino acid mutants of DAD2 had altered interactions with these binding partners. In this study, we tested the mutants in two plant systems, and petunia. Testing in was enabled by creating a CRISPR-Cas9 knockout mutant of the strigolactone receptor (AtD14). We produced SL receptor activity in both systems using wild type and mutant genes; however, the mutants had functions largely indistinguishable from those of the wild type. The expression of the wild type DAD2 from the CaMV 35S promoter in petunia produced plants neither quite like the mutant nor the V26 wild type. These plants had greater height and leaf size although branch number and the plant shape remained more like those of the mutant. These traits may be valuable in the context of a restricted area growing system such as controlled environment agriculture.
矮牵牛独脚金内酯（SL）激素受体DAD2的作用不仅取决于其与PhMAX2A和PhD53A蛋白的相互作用，还取决于其在植物体内的表达模式。此前，在酵母双杂交系统中，我们发现DAD2的一系列单氨基酸和双氨基酸突变体与这些结合伴侣的相互作用发生了改变。在本研究中，我们在两种植物体系（拟南芥和矮牵牛）中对这些突变体进行了测试。通过创建拟南芥独脚金内酯受体（AtD14）的CRISPR-Cas9敲除突变体，我们得以在拟南芥中进行测试。我们使用野生型和突变基因在这两种体系中产生了SL受体活性；然而，这些突变体的功能与野生型的功能在很大程度上难以区分。在矮牵牛中，由CaMV 35S启动子驱动的野生型DAD2的表达所产生的植株既不太像拟南芥突变体，也不太像V26野生型。这些植株更高、叶片更大，尽管分支数量和植株形状更像突变体。在诸如可控环境农业等受限种植区域系统的背景下，这些性状可能具有重要价值。
