Department of Hematology, Center of Expertise for Cancer Survivorship, Radboud University Medical Center, Nijmegen, Netherlands.
Department for Health Evidence, Radboud University Medical Center, Nijmegen, Netherlands.
J Immunol Res. 2023 Oct 20;2023:5863995. doi: 10.1155/2023/5863995. eCollection 2023.
Childhood cancer survivors (CCS) who received radiotherapy involving the spleen or total body irradiation (TBI) might be at risk for splenic dysfunction. A comprehensive screening test for examining splenic dysfunction is lacking.
We investigated whether IgM memory B-cells could be used to assess splenic dysfunction in CCS who received a splenectomy, radiotherapy involving the spleen, or TBI.
All CCS were enrolled from the DCCSS-LATER cohort. We analyzed differences in IgM memory B-cells and Howell-Jolly bodies (HJB) in CCS who had a splenectomy ( = 9), received radiotherapy involving the spleen ( = 36), or TBI ( = 15). IgM memory B-cells < 9 cells/L was defined as abnormal.
We observed a higher median number of IgM memory B-cells in CCS who received radiotherapy involving the spleen (31 cells/L, =0.06) or TBI (55 cells/L, = 0.03) compared to CCS who received splenectomy (20 cells/L). However, only two CCS had IgM memory B-cells below the lower limit of normal. No difference in IgM memory B-cells was observed between CCS with HJB present and absent (35 cells/L vs. 44 cells/L).
Although the number of IgM memory B-cells differed between splenectomized CCS and CCS who received radiotherapy involving the spleen or TBI, only two CCS showed abnormal values. Therefore, this assessment cannot be used to screen for splenic dysfunction.
接受涉及脾脏的放射治疗或全身照射（TBI）的儿童癌症幸存者（CCS）可能存在脾功能障碍的风险。目前缺乏用于检查脾功能障碍的综合筛选测试。
我们研究了 IgM 记忆 B 细胞是否可用于评估接受脾切除术、涉及脾脏的放射治疗或 TBI 的 CCS 的脾功能障碍。
所有 CCS 均从 DCCSS-LATER 队列中招募。我们分析了脾切除术（n=9）、涉及脾脏的放射治疗（n=36）或 TBI（n=15）的 CCS 之间 IgM 记忆 B 细胞和豪-杰氏小体（HJB）的差异。IgM 记忆 B 细胞<9 个/升定义为异常。
与接受脾切除术的 CCS（20 个/升）相比，我们观察到接受涉及脾脏的放射治疗或 TBI 的 CCS 的 IgM 记忆 B 细胞中位数更高（分别为 31 个/升，P=0.06 和 55 个/升，P=0.03）。然而，只有两名 CCS 的 IgM 记忆 B 细胞低于正常下限。有 HJB 和无 HJB 的 CCS 之间的 IgM 记忆 B 细胞无差异（35 个/升比 44 个/升）。
尽管脾切除术和接受涉及脾脏的放射治疗或 TBI 的 CCS 之间的 IgM 记忆 B 细胞数量存在差异，但只有两名 CCS 显示异常值。因此，这种评估不能用于筛选脾功能障碍。
