State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.
University of Science and Technology of China, Anhui 230026, P. R. China.
Biomater Sci. 2023 Dec 5;11(24):7845-7855. doi: 10.1039/d3bm01391j.
Massive bleeding and wound infection due to severe traumas pose a huge threat to the life and health of sufferers; therefore, it is of clinical importance to fabricate adhesives with rapid hemostatic and superior antibacterial capabilities. However, the weak wet adhesion and insufficient function of existing bioadhesives limits their practical application. In this study, a sandcastle worm protein inspired polyelectrolyte self-coacervate adhesive of poly-γ-glutamic acid (PGA) and lysozyme (LZM) was developed. The adhesive exhibited strong underwater adhesion to various surfaces (>250 kPa for solid plates and >50 kPa for soft tissues) and maintained a 80 kPa even when soaked in water for 7 days. Rat liver and tail defect bleeding models revealed that the hemostatic efficiency was superior to that of commercial samples. The antimicrobial tests showed that the bacterial inhibition to and reached almost 100%. Additionally, the infected wound regeneration model demonstrated that the healing rate of the adhesive group was about 100% within 15 days, which was greater than that of the control group. and experiments proved that this facilely prepared adhesive will be a promising material to fulfil the integration functions for rapid wound closure and facilitating wound healing.
由于严重创伤导致的大出血和伤口感染对患者的生命和健康构成了巨大威胁；因此，制备具有快速止血和优异抗菌性能的胶粘剂具有重要的临床意义。然而，现有生物胶粘剂的弱湿黏附性和功能不足限制了其实际应用。在本研究中，开发了一种受沙堡蠕虫蛋白启发的聚γ-谷氨酸（PGA）和溶菌酶（LZM）的聚电解质自凝聚胶粘剂。该胶粘剂对各种表面表现出很强的水下黏附性（固体板>250kPa，软组织>50kPa），即使在水中浸泡 7 天，仍能保持 80kPa 的黏附力。大鼠肝和尾缺损出血模型表明，其止血效率优于商业样本。抗菌试验表明，对 和 的抑菌率几乎达到 100%。此外，感染伤口再生模型表明，在 15 天内，胶粘剂组的愈合率约为 100%，高于对照组。体内实验证明，这种简单制备的胶粘剂将是一种有前途的材料，可以满足快速伤口闭合和促进伤口愈合的综合功能需求。
