Zan Lixia, Zhang Wenyi, Shang Shufeng, Cui Yuanyuan, Pei Jinjin, Yuan Yahong, Yue Tianli
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
College of Biological Sciences and Engineering, Shaanxi Province Key Laboratory of Bio-resources, QinLing-Bashan Mountains Bioresources Comprehensive Development C. I. C., Qinba State Key Laboratory of Biological Resources and Ecological Environment, Shaanxi University of Technology, Hanzhong 723000, China.
Food Funct. 2023 Nov 13;14(22):10151-10162. doi: 10.1039/d3fo03842d.
The aim of this study is to investigate the alleviating effect of selenium-enriched (SL) 6076 on colitis and liver inflammation induced by sodium dextran sulfate (DSS) in mice and its potential molecular mechanisms. (LA) was cultured for 3 generations on MRS medium containing sodium selenite to generate SL. LA (3.2 × 10 CFU mL), low selenium (LS) (3.9 × 10 CFU mL, 0.35 mg mL Se) and high selenium (HS) (2.8 × 10 CFU mL, 0.52 mg mL Se) were continuously fed to mice for 21 d to observe their effects on DSS-induced colitis and liver inflammation in mice. The composition of gut microbiota was detected through high-throughput 16S rRNA sequencing, and inflammatory cytokines, oxidative stress parameters, and serum biochemical indicators were measured in the colon and liver using quantitative polymerase chain reaction (qPCR) and biochemical analysis methods. The results showed that SL alleviated inflammation symptoms in the colon and liver, reduced the expression of inflammatory factors in the colon and liver, regulated oxidative stress responses in the colon, downregulated NF-κB-P65 pathway factors, and altered the composition and structure of the gut microbiota. In summary, DSS-induced colitis may cause liver inflammation, and SL had a significant relieving effect on both colon and liver inflammation. The intervention effect of SL was better than that of LA, while HS was better than LS. SL had a significant alleviating effect on DSS-induced colitis, and may exert its therapeutic effect by downregulating NF-κB-P65 signaling pathways and regulating the structure of intestinal microbiota. This study provides a new approach for the treatment of colitis.
本研究旨在探讨富硒（SL）6076对葡聚糖硫酸钠（DSS）诱导的小鼠结肠炎和肝脏炎症的缓解作用及其潜在分子机制。将乳酸杆菌（LA）在含有亚硒酸钠的MRS培养基上培养3代以生成SL。将LA（3.2×10 CFU/mL）、低硒（LS）（3.9×10 CFU/mL，0.35 mg/mL硒）和高硒（HS）（2.8×10 CFU/mL，0.52 mg/mL硒）连续喂给小鼠21天，以观察它们对DSS诱导的小鼠结肠炎和肝脏炎症的影响。通过高通量16S rRNA测序检测肠道微生物群的组成，并使用定量聚合酶链反应（qPCR）和生化分析方法测量结肠和肝脏中的炎性细胞因子、氧化应激参数和血清生化指标。结果表明，SL减轻了结肠和肝脏的炎症症状，降低了结肠和肝脏中炎性因子的表达，调节了结肠中的氧化应激反应，下调了NF-κB-P65信号通路因子，并改变了肠道微生物群的组成和结构。综上所述，DSS诱导的结肠炎可能会导致肝脏炎症，而SL对结肠和肝脏炎症均有显著缓解作用。SL的干预效果优于LA，而HS优于LS。SL对DSS诱导的结肠炎有显著缓解作用，可能通过下调NF-κB-P65信号通路和调节肠道微生物群结构发挥其治疗作用。本研究为结肠炎的治疗提供了一种新方法。
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