Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia E-mail:
Graduate School of BioMedical Engineering, University of New South Wales, Sydney 2052, Australia.
J Water Health. 2023 Oct;21(10):1580-1590. doi: 10.2166/wh.2023.217.
Cryptosporidium spp. are protozoan parasites of significant health importance found in environmental waters globally. Four commercially available Cryptosporidium-specific immunomagnetic separation (IMS) kits used in various water sample matrices were analysed and compared. Beads were characterised by flow cytometry and tested for the recovery efficiencies for oocysts spiked into different matrices: river water sediment, clay sample, and filter backwash sample. Results showed that Dynabeads™ Cryptosporidium and Waterborne Crypto-Grab™ kits contained immunoglobulin IgM antibody-coated beads. In contrast, the BioPoint CryptoBead and the TCS Isolate kits contained immunoglobulin IgG antibody-coated beads. BioPoint CryptoBead was significantly coated with more antibodies and were able to capture oocysts more rapidly compared to the other beads. Recovery efficiencies of Dynabeads™, TCS Isolate® beads, and BioPoint CryptoBead ranged from 55 to 93% when tested against different sample matrices, with BioPoint CryptoBead resulting in the highest at 93% in reagent-grade water and Dynabeads™ at 55%, the lowest against clay samples. The Waterborne beads did not perform well on any samples, with recovery efficiencies ranging from 0 to 8%. Fluorescence microscopy analyses showed that both the IMS method and the sample matrix processed affect the quality of the membranes, with the cleanest samples for microscopy examination observed from BioPoint CryptoBead.
隐孢子虫是一种具有重要健康意义的原生动物寄生虫，在全球环境水中都有发现。分析并比较了四种可商购的用于各种水样基质的隐孢子虫特异性免疫磁分离（IMS）试剂盒。通过流式细胞术对珠子进行了表征，并测试了将卵囊掺入不同基质（河水沉积物、粘土样品和滤池反冲洗样品）中的回收率效率。结果表明，Dynabeads™隐孢子虫和 Waterborne Crypto-Grab™试剂盒中含有免疫球蛋白 IgM 抗体包被的珠子。相比之下，BioPoint CryptoBead 和 TCS Isolate 试剂盒中含有免疫球蛋白 IgG 抗体包被的珠子。与其他珠子相比，BioPoint CryptoBead 明显包被了更多的抗体，并且能够更快地捕获卵囊。在测试不同的样品基质时，Dynabeads™、TCS Isolate®珠子和 BioPoint CryptoBead 的回收率效率范围为 55%至 93%，其中 BioPoint CryptoBead 在试剂级水中的回收率最高，为 93%，在粘土样品中的回收率最低，为 55%。Waterborne 珠子在任何样品上的性能都不佳，回收率效率范围为 0%至 8%。荧光显微镜分析表明，IMS 方法和处理的样品基质都影响膜的质量，通过 BioPoint CryptoBead 观察到显微镜检查中最干净的样品。
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