Pennsylvania Animal Diagnostic Laboratory System Toxicology Laboratory, New Bolton Center, University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pennsylvania, USA.
J Sep Sci. 2024 Jan;47(1):e2300630. doi: 10.1002/jssc.202300630. Epub 2023 Oct 30.
Hemp-based materials have gained interest as alternative feed ingredients for livestock. However, safety concerns arise regarding the transfer of cannabinoids from the plant to the animals. Addressing these concerns requires the use of methods capable of detecting and quantifying cannabinoids in livestock. In this study, a fast and sensitive method was developed for quantification of cannabinoids and cannabinoid metabolites in cattle plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The extraction of cannabinoids from the plasma matrix was achieved by combining the Captiva Enhanced Matrix Removal-Lipid clean-up and salting-out assisted liquid-liquid extraction procedure. The developed method underwent validation using various analytical parameters, and the results demonstrated good accuracy, precision, specificity, and high sensitivity. The method was applied to real plasma samples obtained from cattle fed hemp for 2 weeks, and successfully detected various cannabinoids, including delta-9-tetrahydrocannabinol. Furthermore, the study revealed that 7-carboxy cannabidiol, a metabolite of cannabidiol, was the predominant cannabinoid present in the cattle plasma throughout the feeding period, which could remain detectable for weeks after the hemp feeding had ended.
麻基材料作为家畜的替代饲料成分引起了人们的兴趣。然而，人们对大麻素从植物转移到动物的安全性表示担忧。解决这些问题需要使用能够检测和定量牲畜中大麻素的方法。在这项研究中，开发了一种使用液相色谱-串联质谱（LC-MS/MS）定量牛血浆中大麻素和大麻素代谢物的快速灵敏方法。通过结合 Captiva 增强基质去除-脂质净化和盐析辅助液-液萃取程序，从血浆基质中提取大麻素。该方法经过各种分析参数的验证，结果表明具有良好的准确性、精密度、特异性和高灵敏度。该方法应用于从喂食大麻两周的牛获得的实际血浆样品中，并成功检测到各种大麻素，包括 delta-9-四氢大麻酚。此外，该研究表明，7-羧酸大麻二酚是大麻二酚的代谢物，是整个喂养期间牛血浆中存在的主要大麻素，在大麻喂养结束后数周仍可检测到。
