Department of Animal Sciences, North Dakota State University, Fargo, ND, USA.
USDA-Agricultural Research Service, Edward T. Schafer Agricultural Research Center, Biosciences Research Laboratory, Fargo, ND, USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2022 Oct;39(10):1705-1717. doi: 10.1080/19440049.2022.2107711. Epub 2022 Aug 8.
Hempseed cake is a byproduct of hempseed oil extraction and is potentially a useful source of protein and fiber for use in ruminant diets. However, data are lacking on the appearance and/or clearance of cannabinoids in tissues of animals fed hempseed cake. To this end, a rapid method for quantifying cannabinol (CBN), cannabidiol (CBD), cannabinolic acid (CBNA), cannabidiolic acid (CBDA), cannabigerolic acid (CBGA), cannabichromenic acid (CBCA), cannabidivarin (CBDV), cannabidivarinic acid (CBDVA), tetrahydrocannabinol (THC) and tetrahydrocannabinolic acid (THCA) in cattle tissues, plasma, and urine was developed using rapid screen electrospray ionization mass spectrometry (RS-ESI-MS). Regression coefficients of matrix-matched standard curves ranged from 0.9946 to >0.9999 and analyte recoveries averaged from 90.2 ± 15.5 to 108.7 ± 18.7% across all compounds. Limits of detection and quantification ranged from 0.05 to 2.79 ng · mL and 0.17 to 9.30 ng · mL, respectively, while the inter-day relative standard deviation ranged from 5.1 to 15.1%. Rapid screening electrospray ionization mass spectrometry (RS-ESI-MS) returned no false positives for any cannabinoid in plasma, urine, and tissue (liver, skeletal muscle) samples from 6 non-dosed control animals ( = 90 samples; of which 72 samples were plasma or urine and 18 samples were tissues). Across-animal cannabinoid concentrations measured in 32 plasma samples of cattle dosed with ground hemp were quantified by RS-ESI-MS; analytical results correlated well ( = 0.963) with independent LC-MS/MS analysis of the same samples.
麻籽饼是麻籽油提取的副产品，可能是反刍动物饲料中有用的蛋白质和纤维来源。然而，关于饲喂麻籽饼的动物组织中大麻素的外观和/或清除的数据尚缺乏。为此，开发了一种快速定量分析牛肉组织、血浆和尿液中大麻酚（CBN）、大麻二酚（CBD）、大麻酸（CBNA）、大麻二酚酸（CBDA）、大麻萜酚酸（CBGA）、大麻素酸（CBCA）、大麻二酚（CBDV）、大麻二酚酸（CBDVA）、四氢大麻酚（THC）和四氢大麻酚酸（THCA）的快速筛选电喷雾电离质谱法（RS-ESI-MS）。基质匹配标准曲线的回归系数范围为 0.9946 至>0.9999，所有化合物的分析物回收率平均为 90.2±15.5%至 108.7±18.7%。检测限和定量限范围分别为 0.05 至 2.79ng·mL和 0.17 至 9.30ng·mL，而日间相对标准偏差范围为 5.1%至 15.1%。快速筛选电喷雾电离质谱法（RS-ESI-MS）在 6 只未给药对照动物（=90 个样本；其中 72 个样本为血浆或尿液，18 个样本为组织）的血浆、尿液和组织（肝脏、骨骼肌）样本中对任何大麻素均未返回假阳性。32 份经地面麻籽处理的牛血浆样本的跨动物大麻素浓度通过 RS-ESI-MS 进行定量；分析结果与相同样本的独立 LC-MS/MS 分析相关性良好（=0.963）。
