Huzhou Central Hospital, Affiliated Central Hospital Huzhou University, Huzhou, Zhejiang 313000, China.
Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou, Huzhou, Zhejiang 313000, China.
Nucleic Acids Res. 2024 Jan 5;52(D1):D1033-D1041. doi: 10.1093/nar/gkad955.
The brain is constituted of heterogeneous types of neuronal and non-neuronal cells, which are organized into distinct anatomical regions, and show precise regulation of gene expression during development, aging and function. In the current database release, STAB2 provides a systematic cellular map of the human and mouse brain by integrating recently published large-scale single-cell and single-nucleus RNA-sequencing datasets from diverse regions and across lifespan. We applied a hierarchical strategy of unsupervised clustering on the integrated single-cell transcriptomic datasets to precisely annotate the cell types and subtypes in the human and mouse brain. Currently, STAB2 includes 71 and 61 different cell subtypes defined in the human and mouse brain, respectively. It covers 63 subregions and 15 developmental stages of human brain, and 38 subregions and 30 developmental stages of mouse brain, generating a comprehensive atlas for exploring spatiotemporal transcriptomic dynamics in the mammalian brain. We also augmented web interfaces for querying and visualizing the gene expression in specific cell types. STAB2 is freely available at https://mai.fudan.edu.cn/stab2.
大脑由多种类型的神经元和非神经元细胞组成，这些细胞组织成不同的解剖区域，并在发育、衰老和功能过程中表现出精确的基因表达调控。在当前的数据库版本中，STAB2 通过整合来自不同区域和整个生命周期的大规模单细胞和单核 RNA 测序数据集，为人类和小鼠大脑提供了系统的细胞图谱。我们应用无监督聚类的分层策略对整合的单细胞转录组数据集进行分析，以精确注释人类和小鼠大脑中的细胞类型和亚型。目前，STAB2 分别包含了人类和小鼠大脑中 71 种和 61 种不同的细胞亚型。它涵盖了人类大脑的 63 个亚区和 15 个发育阶段，以及小鼠大脑的 38 个亚区和 30 个发育阶段，为探索哺乳动物大脑中的时空转录组动态提供了一个全面的图谱。我们还增强了用于查询和可视化特定细胞类型中基因表达的网络界面。STAB2 可在 https://mai.fudan.edu.cn/stab2 上免费获取。
