Center for Nanomedicine, Institute for Basic Science, Yonsei University, Seoul, 03722, Republic of Korea.
Graduate Program in Nanobiomedical Engineering, Advanced Science Institute, Yonsei University, Seoul, 03722, Republic of Korea.
Adv Sci (Weinh). 2023 Jun;10(16):e2206939. doi: 10.1002/advs.202206939. Epub 2023 Apr 7.
Spatial transcriptomics is a newly emerging field that enables high-throughput investigation of the spatial localization of transcripts and related analyses in various applications for biological systems. By transitioning from conventional biological studies to "in situ" biology, spatial transcriptomics can provide transcriptome-scale spatial information. Currently, the ability to simultaneously characterize gene expression profiles of cells and relevant cellular environment is a paradigm shift for biological studies. In this review, recent progress in spatial transcriptomics and its applications in neuroscience and cancer studies are highlighted. Technical aspects of existing technologies and future directions of new developments (as of March 2023), computational analysis of spatial transcriptome data, application notes in neuroscience and cancer studies, and discussions regarding future directions of spatial multi-omics and their expanding roles in biomedical applications are emphasized.
空间转录组学是一个新兴领域，能够在各种生物系统的应用中进行高通量的转录本空间定位研究和相关分析。通过从传统的生物学研究过渡到“原位”生物学，空间转录组学可以提供转录组规模的空间信息。目前，能够同时描述细胞的基因表达谱和相关细胞环境的能力是生物学研究的一个范式转变。在这篇综述中，强调了空间转录组学的最新进展及其在神经科学和癌症研究中的应用。突出了现有技术的技术方面和新发展的未来方向（截至 2023 年 3 月）、空间转录组数据的计算分析、神经科学和癌症研究中的应用说明，以及关于空间多组学的未来方向及其在生物医学应用中的扩展作用的讨论。
