盘基网柄菌的尿嘧啶-DNA糖基化酶活性
Uracil-DNA glycosylase activity from Dictyostelium discoideum.
作者信息
Guyer R B, Nonnemaker J M, Deering R A
出版信息
Biochim Biophys Acta. 1986 Dec 18;868(4):262-4. doi: 10.1016/0167-4781(86)90063-1.
We have isolated and partially characterized a uracil-DNA glycosylase activity from the cellular slime mold, Dictyostelium discoideum. This glycosylase has a broad pH optimum (6.5-8.5) and is fully active in 10 mM EDTA or in 5 mM Mg2+. Its molecular weight by gel filtration is about 55 000. This enzyme activity may work in concert with previously described apurinic/apyrimidinic (AP) endonuclease activities in the excision repair of uracil from the DNA of this lower eukaryote.
我们已经从细胞黏菌盘基网柄菌中分离出一种尿嘧啶-DNA糖基化酶活性，并对其进行了部分特性分析。这种糖基化酶具有较宽的最适pH值（6.5 - 8.5），在10 mM乙二胺四乙酸（EDTA）或5 mM镁离子（Mg2+）中能完全发挥活性。通过凝胶过滤法测得其分子量约为55000。在这种低等真核生物的DNA中，该酶活性可能与先前描述的脱嘌呤/脱嘧啶（AP）内切酶活性协同作用，参与尿嘧啶的切除修复。
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