Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang 315211, China.
Analyst. 2023 Nov 20;148(23):5864-5872. doi: 10.1039/d3an01473h.
A hydrophilic phosphoserine-functionalized magnetic organic framework composite (termed FeO@COF@MOF-PS) was synthesized by an growth strategy for effective capture of -glycopeptides. FeO@COF@MOF-PS exhibited high sensitivity (0.2 fmol μL), outstanding exclusion of size capability (1 : 10 000), good selectivity (1 : 2000), and reusability (at least 10 times). It also exhibited remarkable performance in the -glycopeptide analysis in complex biological samples. nano-LC-MS/MS analysis, a total of 223 -glycopeptides with 161 glycosylation sites assigned to 91 glycoproteins and 331 -glycopeptides with 243 glycosylation sites assigned to 134 glycoproteins were identified in sera from cervical cancer patients and normal controls, respectively. Biological processes and molecular functional analyses indicate that the captured glycoproteins are of significant relevance to cervical cancer, for example, gene coverage or expression of cell adhesion and extracellular matrix structural constituents. Thus, FeO@COF@MOF-PS not only efficiently captures -glycopeptides, but also has the possibility of screening potential disease markers and elucidating the process of cervical cancer development.
一种亲水磷酸丝氨酸功能化磁性有机骨架复合材料（称为 FeO@COF@MOF-PS）通过一种 生长策略合成，用于有效捕获 -糖肽。FeO@COF@MOF-PS 表现出高灵敏度（0.2 fmol μL）、出色的排除大小能力（1：10000）、良好的选择性（1：2000）和可重复使用性（至少 10 次）。它还在复杂生物样品中的 -糖肽分析中表现出出色的性能。通过 nano-LC-MS/MS 分析，分别从宫颈癌患者和正常对照者的血清中鉴定出 223 种带有 161 个糖基化位点的 -糖肽，分配到 91 种糖蛋白中，以及 331 种带有 243 个糖基化位点的 -糖肽，分配到 134 种糖蛋白中。生物过程和分子功能分析表明，捕获的糖蛋白与宫颈癌具有显著相关性，例如细胞黏附的基因覆盖率或表达以及细胞外基质结构成分。因此，FeO@COF@MOF-PS 不仅可以有效地捕获 -糖肽，还有可能筛选潜在的疾病标志物并阐明宫颈癌发展的过程。
