sp. nov.，分离自智利养殖的大西洋鲑（L.）。
sp. nov., isolated from Atlantic salmon ( L.) cultured in Chile.
机构信息
Universidad Andrés Bello, Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias de la Vida, Viña del Mar, Chile.
Interdisciplinary Center for Aquaculture Research (INCAR), Viña del Mar, Chile.
出版信息
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006102.
Strain PVT-9a, a novel Gram-stain-negative, aerobic, non-spore-forming, motile-by-gliding and rod-shaped bacterium, was isolated from a skin lesion of Atlantic salmon ( L.) during a tenacibaculosis outbreak that occurred in 2016 at a Chilean fish farm. Phylogenetic analysis based on 16S rRNA gene sequencing confirmed that strain PVT-9a belonged to the genus , being related to the closest type strains KCTC 52419 (98.49 % sequence similarity), JDTF-79 (97.36 %), JDTF-31 (97.29 %) and IFO 15947 (97.15 %). The genome size of strain PVT-9a was 2.73 Mb with a DNA G+C content 31.09 mol%. Average nucleotide identity analysis among 30 species rendered the most similar strains as follows: KCTC 52419 (87.91 %), IFO 15947 (82.47 %), 35/09 (81.08 %), gv TNO006 (80.91 %) and gv TNO010 (80.96 %). Menaquinone MK-6 was the predominant respiratory quinone. The predominant cell fatty acids (>10 %) were iso-C, iso-C G and iso-C 3-OH. Phenotypic, chemotaxonomic and genomic data supported the assignment of strain PVT-9a (=DSM 115155=RGM 3472) as representing a novel species of , for which the name sp. nov. is proposed.
菌株 PVT-9a 是一株革兰氏阴性、需氧、非孢子形成、滑行运动的杆状细菌，从 2016 年智利一家鱼场爆发的弧菌病中大西洋鲑鱼（L.）的皮肤损伤中分离得到。基于 16S rRNA 基因测序的系统发育分析证实，菌株 PVT-9a 属于 属，与最接近的模式菌株 KCTC 52419（98.49%序列相似性）、JDTF-79（97.36%）、JDTF-31（97.29%）和 IFO 15947（97.15%）最为相似。菌株 PVT-9a 的基因组大小为 2.73 Mb，G+C 含量为 31.09 mol%。在 30 个种属的平均核苷酸同一性分析中，最相似的菌株如下：KCTC 52419（87.91%）、IFO 15947（82.47%）、35/09（81.08%）、gv TNO006（80.91%）和 gv TNO010（80.96%）。menaquinone MK-6 是主要的呼吸醌。主要的细胞脂肪酸（>10%）是 iso-C、iso-C G 和 iso-C 3-OH。表型、化学分类和基因组数据支持菌株 PVT-9a（=DSM 115155=RGM 3472）被归为一个新的属，建议将其命名为 sp. nov.。
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