Universidad Andrés Bello, Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias de la Vida, Viña del Mar, Chile.
Interdisciplinary Center for Aquaculture Research (INCAR), Viña del Mar, Chile.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006468.
Strain T-12, an orange, Gram-stain-negative, non-motile, rod-shaped strain, was isolated in November 2013 from water samples collected from an Atlantic salmon () fry culturing system at a fish farm in Chile. Phylogenetic analysis based on 16S rRNA sequences (1394 bp) revealed that strain T-12 belonged to the genus , showing close relationships to F-372 (99.48 %) and DS-20 (98.50 %). The genome size of strain T-12 was 3.28 Mb, with a G+C content of 31.1 mol%. Genome comparisons aligned strain T-12 with F-372 (GCA_011305415) and DSM 17934 (GCA_900108955). The highest digital DNA-DNA hybridization (dDDH) values were 42.6 % with F-372 (GCA_011305415) and 33.9 % with DSM 17934 (GCA_900108955). Pairwise average nucleotide identity (ANI) calculations were below the species cutoff, with the best results with F-372 being: ANIb, 90.33 %; ANIm, 91.85 %; and TETRA, 0.997 %. These dDDH and ANI results confirm that strain T-12 represents a new species. The major fatty acids were iso-C and Cω6. Detected polar lipids included phospholipids (=2), aminophospholipid (=1), aminolipid (=1) and unidentified lipids (=2). The predominant respiratory quinone was menaquinone MK7 (80 %) followed by MK-6 (20 %). Phenotypic, chemotaxonomic, and genomic data support the classification of strain T-12 (=CECT 30410=RGM 3222) as representing a novel species of , for which the name sp. nov. is proposed.
T-12 菌株，呈橙色，革兰氏阴性，不运动，杆状，于 2013 年 11 月从智利一个鱼类养殖场大西洋鲑鱼鱼苗养殖系统采集的水样中分离得到。基于 16S rRNA 序列（1394bp）的系统发育分析显示，菌株 T-12 属于 属，与 F-372（99.48%）和 DS-20（98.50%）关系密切。菌株 T-12 的基因组大小为 3.28Mb，G+C 含量为 31.1mol%。基因组比较将菌株 T-12 与 F-372（GCA_011305415）和 DSM 17934（GCA_900108955）对齐。最高的数字 DNA-DNA 杂交（dDDH）值分别为 42.6%与 F-372（GCA_011305415）和 33.9%与 DSM 17934（GCA_900108955）。成对平均核苷酸同一性（ANI）计算低于种间截止值，与 F-372 的最佳结果为：ANIb，90.33%；ANIm，91.85%；和 TETRA，0.997%。这些 dDDH 和 ANI 结果证实，菌株 T-12 代表一个新的种。主要脂肪酸为 iso-C 和 Cω6。检测到的极性脂包括磷脂（=2）、氨基磷脂（=1）、氨基脂（=1）和未鉴定的脂（=2）。主要呼吸醌为menaquinone MK7（80%），其次为 MK-6（20%）。表型、化学分类和基因组数据支持菌株 T-12（=CECT 30410=RGM 3222）被归类为 属的一个新种，建议命名为 sp. nov.。
