Department of Molecular Neurobiology and Institute for Developmental Research, Aichi Human Service Center, Kasugai, Japan.
Cellular Pathology, Institute for Developmental Research, Aichi Developmental Disability Center, Kasugai, Japan.
Dev Neurosci. 2024;46(4):262-272. doi: 10.1159/000534886. Epub 2023 Oct 31.
C-terminal-binding protein 1 (CtBP1) is a multi-functional protein with well-established roles as a transcriptional co-repressor in the nucleus and a regulator of membrane fission in the cytoplasm. Although CtBP1 gene abnormalities have been reported to cause neurodevelopmental disorders, the physiological role and expression profile of CtBP1 remains to be elucidated.
In this study, we used biochemical, immunohistochemical, and immunofluorescence methods to analyze the expression of CtBP1 during mouse brain development.
Western blotting analyses revealed that CtBP1 appeared to be expressed mainly in the central nervous system throughout the developmental process. In immunohistochemical analyses, region-specific nuclear as well as weak cytoplasmic distribution of CtBP1 was observed in telencephalon at embryonic day (E)15 and E17. It is of note that CtBP1 was barely detected in axons but observed in the nucleus of oligodendrocytes in the white matter at E17. As to the cerebellum at postnatal day 30, CtBP1 appeared to be expressed in the nucleus and cytoplasm of Purkinje cells, the nucleus of granule cells and cells in the molecular layer (ML), and the ML per se, where granule cell axons and Purkinje cell dendrites are enriched. In addition, CtBP1 was detected in the cerebellar nuclei.
The obtained results suggest involvement of CtBP1 in brain function.
C 端结合蛋白 1（CtBP1）是一种多功能蛋白，在核内作为转录共抑制因子和在细胞质中作为膜裂变调节剂的作用已得到充分证实。尽管已经报道 CtBP1 基因异常会导致神经发育障碍，但 CtBP1 的生理作用和表达谱仍有待阐明。
在这项研究中，我们使用生化、免疫组织化学和免疫荧光方法分析了 CtBP1 在小鼠大脑发育过程中的表达。
Western blot 分析表明，CtBP1 在整个发育过程中似乎主要在中枢神经系统中表达。在免疫组织化学分析中，在胚胎期 15 天（E15）和 17 天（E17）的端脑中观察到 CtBP1 的区域特异性核内以及弱细胞质分布。值得注意的是，CtBP1 在轴突中几乎检测不到，但在白质中的少突胶质细胞的核中观察到。至于 30 天大的小脑，CtBP1 似乎在浦肯野细胞的核和细胞质、颗粒细胞的核和分子层（ML）中的细胞以及富含颗粒细胞轴突和浦肯野细胞树突的 ML 本身中表达。此外，CtBP1 也在小脑核中被检测到。
这些结果表明 CtBP1 参与了大脑功能。
