Harris Orthopaedic Laboratory, Massachusetts General Hospital, Boston, MA, USA.
Department of Orthopaedic Surgery, Harvard Medical School, 55 Fruit St. GRJ 1231, Boston, MA, 02114, USA.
BMC Musculoskelet Disord. 2023 Oct 31;24(1):854. doi: 10.1186/s12891-023-06942-5.
Rodent models are commonly employed to validate preclinical disease models through the evaluation of postoperative behavior and allodynia. Our study investigates the dynamic interplay between pain and functional recovery in the context of traumatic osteotomy and surgical repair. Specifically, we established a rat model of tibial osteotomy, followed by internal fixation using a 5-hole Y-plate with 4 screws, to explore the hypothesis that histological bone healing is closely associated with functional recovery.
Our primary objective was to assess the correlation between bone healing and functional outcomes in a rat model of tibial osteotomy and plate fixation.
Seventeen male Sprague-Dawley rats underwent a metaphyseal transverse osteotomy of the proximal tibia, simulating a fracture-like injury. The resultant bone defect was meticulously repaired by realigning and stabilizing the bone surfaces with the Y-plate. To comprehensively assess recovery and healing, we performed quantitative and qualitative evaluations at 2, 4, 6, and 8 weeks post-surgery. Evaluation methods included micro-CT imaging, X-ray analysis, and histological examination to monitor bone defect healing. Concurrently, we employed video recording and gait analysis to evaluate functional recovery, encompassing parameters such as temporal symmetry, hindlimb duty factor imbalance, phase dispersion, and toe spread.
Our findings revealed complete healing of the bone defect at 8 weeks, as confirmed by micro-CT and histological assessments. Specifically, micro-CT data showed a decline in fracture volume over time, indicating progressive healing. Histological examination demonstrated the formation of new trabecular bone and the resolution of inflammation. Importantly, specific gait analysis parameters exhibited longitudinal changes consistent with bone healing. Hindlimb duty factor imbalance, hindlimb temporal symmetry, and phase dispersion correlated strongly with the healing process, emphasizing the direct link between bone healing and functional outcomes.
The establishment of this tibia osteotomy model underscores the association between bone healing and functional outcomes, emphasizing the feasibility of monitoring postoperative recovery using endpoint measurements. Our overarching objective is to employ this model for assessing the local efficacy of drug delivery devices in ameliorating post-surgical pain and enhancing functional recovery.
啮齿动物模型常用于通过评估术后行为和痛觉过敏来验证临床前疾病模型。我们的研究调查了创伤性骨折和手术修复背景下疼痛和功能恢复之间的动态相互作用。具体来说，我们建立了一种大鼠胫骨骨折模型，然后使用带有 4 个螺钉的 5 孔 Y 板进行内固定，以探讨假设即组织学骨愈合与功能恢复密切相关。
我们的主要目的是评估胫骨骨折和钢板固定大鼠模型中骨愈合与功能结果之间的相关性。
17 只雄性 Sprague-Dawley 大鼠接受胫骨近端干骺端横断骨折手术，模拟骨折样损伤。通过 Y 形板对线和稳定骨表面，精心修复由此产生的骨缺损。为了全面评估恢复和愈合情况，我们在术后 2、4、6 和 8 周进行了定量和定性评估。评估方法包括微 CT 成像、X 射线分析和组织学检查，以监测骨缺损愈合。同时，我们采用视频记录和步态分析评估功能恢复，包括时间对称性、后肢责任因子失衡、相位分散和趾距等参数。
我们的研究结果表明，在 8 周时，骨缺损完全愈合，微 CT 和组织学评估均得到证实。具体而言，微 CT 数据显示骨折体积随时间逐渐减少，表明愈合过程在不断进行。组织学检查显示新的小梁骨形成和炎症消退。重要的是，特定的步态分析参数显示出与骨愈合相一致的纵向变化。后肢责任因子失衡、后肢时间对称性和相位分散与愈合过程密切相关，强调了骨愈合与功能结果之间的直接联系。
建立这种胫骨骨折模型突出了骨愈合与功能结果之间的关联，强调了使用终点测量监测术后恢复的可行性。我们的总体目标是利用该模型评估药物输送装置在减轻术后疼痛和促进功能恢复方面的局部疗效。
