Vu Bich Ngoc, Vu Tien Van, Yoo Jae Yong, Nguyen Ngan Thi, Ko Ki Seong, Kim Jae-Yean, Lee Kyun Oh
Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju, Republic of Korea.
Division of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju, Republic of Korea.
Front Plant Sci. 2023 Oct 16;14:1271368. doi: 10.3389/fpls.2023.1271368. eCollection 2023.
Plants consistently encounter environmental stresses that negatively affect their growth and development. To mitigate these challenges, plants have developed a range of adaptive strategies, including the unfolded protein response (UPR), which enables them to manage endoplasmic reticulum (ER) stress resulting from various adverse conditions. The CRISPR-Cas system has emerged as a powerful tool for plant biotechnology, with the potential to improve plant tolerance and resistance to biotic and abiotic stresses, as well as enhance crop productivity and quality by targeting specific genes, including those related to the UPR. This review highlights recent advancements in UPR signaling pathways and CRISPR-Cas technology, with a particular focus on the use of CRISPR-Cas in studying plant UPR. We also explore prospective applications of CRISPR-Cas in engineering UPR-related genes for crop improvement. The integration of CRISPR-Cas technology into plant biotechnology holds the promise to revolutionize agriculture by producing crops with enhanced resistance to environmental stresses, increased productivity, and improved quality traits.
植物不断面临对其生长和发育产生负面影响的环境压力。为了应对这些挑战，植物已经发展出一系列适应性策略，包括未折叠蛋白反应（UPR），这使它们能够应对由各种不利条件导致的内质网（ER）应激。CRISPR-Cas系统已成为植物生物技术的强大工具，通过靶向特定基因，包括与UPR相关的基因，具有提高植物对生物和非生物胁迫的耐受性和抗性的潜力，以及提高作物产量和品质。本综述重点介绍了UPR信号通路和CRISPR-Cas技术的最新进展，特别关注CRISPR-Cas在研究植物UPR中的应用。我们还探讨了CRISPR-Cas在改造与UPR相关基因以改良作物方面的潜在应用。将CRISPR-Cas技术整合到植物生物技术中有望通过培育出对环境胁迫具有更强抗性、更高产量和更优良品质性状的作物来彻底改变农业。
