Postgraduate Program in Dentistry (PPGO) at UNIGRANRIO, Street Prof. José de Souza Herdy, 1,160, block C, 2nd floor - 25th of August - Duque de Caxias - Rio de Janeiro, Brazil. Zip code 25071-202.
Laboratory of Powder Technology, Division of Materials, National Institute of Technology, N° 82 Venezuela Avenue, Room 602, Zip code 20081-312, Rio de Janeiro, RJ, Brazil.
Braz Dent J. 2023 Jul-Aug;34(4):72-84. doi: 10.1590/0103-6440202305353.
This study aimed to evaluate the microstructure formed after the chemical treatment of teeth, for the development of autogenous grafts from the demineralized dentin matrix (DDM) technique, in order to identify the most efficient demineralizing solution. The specimens, originating from the root and coronal portion, were submitted to ultrasonic cleaning and drying in an oven for 1h at 100 ºC. Then, the density was determined by Archimedes' principle for each specimen, using distilled water as immersion liquid. The samples were separated into five groups: Control group: negative control, Distilled water;EDTA group: positive control, trisodium EDTA; NaOCl group: 2.5% sodium hypochlorite; HCl-0.6M group: 0.6M hydrochloric acid; and H2O2/H2SO4 group: hydrogen peroxide and sulfuric acid. Each specimen was immersed for 1h in the corresponding group descaling solution at 60 ºC. Subsequently, the mass loss and density of the treated specimens were determined by Archimedes' principle. Ultimately, the specimens of each group were characterized by microtomography, Scanning Electron Microscopy, and Energy Dispersive Spectrometry X-ray (SEM-EDS). The results demonstrated that the H2O2/H2SO4 solution allowed the formation of interconnected micropores, suggesting better pore structures for application in scaffolds, when compared to the other studied solutions.
本研究旨在评估牙齿化学处理后的微观结构，以开发源自脱矿质牙本质基质 (DDM) 技术的自体移植物，从而确定最有效的脱矿溶液。从根和冠部分离的样本，经过超声清洗和在 100°C 的烘箱中干燥 1 小时。然后，使用蒸馏水作为浸液，通过阿基米德原理确定每个样本的密度。样本分为五组：对照组：负对照，蒸馏水；EDTA 组：正对照，三钠 EDTA；次氯酸钠组：2.5%次氯酸钠；0.6M 盐酸组：0.6M 盐酸；和 H2O2/H2SO4 组：过氧化氢和硫酸。每个样本在 60°C 的相应脱矿溶液中浸泡 1 小时。随后，通过阿基米德原理确定处理后样本的质量损失和密度。最后，通过微断层扫描、扫描电子显微镜和能谱 X 射线 (SEM-EDS) 对每组样本进行了特征描述。结果表明，与其他研究的溶液相比，H2O2/H2SO4 溶液能够形成相互连接的微孔，表明其具有更好的孔结构，适用于支架。
