The Key Laboratory of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, P. R. China.
Comprehensive Technology Center of Zhangjiagang Customs, Zhangjiagang 215600, Jiangsu, P. R. China.
Anal Chem. 2023 Nov 14;95(45):16639-16648. doi: 10.1021/acs.analchem.3c03019. Epub 2023 Nov 1.
The simultaneous detection of multiple antibiotic residues in food is of great significance for food safety. In this work, a novel dual-potential electrochemiluminescence (ECL) immunoassay was designed for the simultaneous detection of chloramphenicol and fluorfenicol residues in food. Ru@MOF was used as an anodic probe, and SnS QDs-PEI-Au-MoS was used as a cathodic probe. Notably, the coreactant for both luminophores was KSO, avoiding interactions caused by different kinds of coreactants. Au nanoparticles functionalized with a nitrogen- and sulfur-doped graphene oxide-modified glassy carbon electrode to improve the electron transfer efficiency and provide a larger surface area for immobilization of antigen. The linear range for the detection of florfenicol was determined to be 0.1-1000 ng mL with a detection limit of 0.03 ng mL, and the linear range for the detection of chloramphenicol was 0.01-1000 ng mL with a detection limit of 3.2 pg mL by recording the ECL responses at two different excitation potentials. The proposed immunoassay achieved a more stable recovery in the detection of actual samples and provided a new analytical method for the simultaneous detection of florfenicol and chloramphenicol residues with high sensitivity and specificity.
同时检测食品中的多种抗生素残留对于食品安全具有重要意义。在这项工作中，设计了一种新颖的双重电化学发光（ECL）免疫分析方法，用于同时检测食品中氯霉素和氟苯尼考残留。Ru@MOF 被用作阳极探针，SnS QDs-PEI-Au-MoS 被用作阴极探针。值得注意的是，两种发光体的共反应物均为 KSO，避免了不同共反应物引起的相互作用。金纳米粒子功能化的氮和硫掺杂氧化石墨烯修饰玻碳电极提高了电子转移效率，并为抗原的固定提供了更大的表面积。通过在两个不同的激发电位下记录 ECL 响应，确定了氟苯尼考的检测线性范围为 0.1-1000 ng mL，检测限为 0.03 ng mL，氯霉素的检测线性范围为 0.01-1000 ng mL，检测限为 3.2 pg mL。所提出的免疫分析在实际样品检测中实现了更稳定的回收率，并提供了一种用于同时检测高灵敏度和特异性的氟苯尼考和氯霉素残留的新分析方法。
