Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.
Department of Life Science, Chosun University, Gwangju 61452, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006099.
Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25-45 °C (optimum, 35 °C); pH 6-9 (optimum, pH 6); and in the presence of 0-6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108 forms a phyletic lineage distinct from other closely related species within the genus . It was found to be most closely related to ATCC 25577 (98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108 was 63.2 mol%. The DNA-DNA hybridization values of strain CBA3108 with ATCC 25577, WCA-380-WT-3A and subsp. DSM 1897 were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is CBA3108 (=KACC 22889=JCM 35966).
CBA3108 菌株是一株从韩国济州岛马粪便样本中分离得到的革兰氏阳性、非孢子形成、专性厌氧菌。CBA3108 细胞为短杆状,无动力,被鉴定为过氧化氢酶阳性和氧化酶阴性。该菌株的生长条件为:25-45℃(最适温度 35℃);pH 值 6-9(最适 pH 值 6);并且在 0-6%(w/v)NaCl(最适 2%)存在的情况下。该菌株的主要脂肪酸包括 Ciso 和 CisoDMA,而主要极性脂包括磷脂酰甘油、双磷脂酰甘油和磷脂酰胆碱。基于 16S rRNA 基因序列的系统发育分析,CBA3108 菌株与属内其他密切相关的种形成一个独特的进化枝。它与 ATCC 25577(98.27%的 16S rRNA 基因序列相似性)和属内的其他菌株(≤98.0%)最为密切相关。CBA3108 菌株的基因组 DNA G+C 含量为 63.2mol%。CBA3108 菌株与 ATCC 25577、WCA-380-WT-3A 和 subsp. DSM 1897 的 DNA-DNA 杂交值分别为 33.6%、21.7%和 22.7%。其表型、化学分类和分子特性支持了 CBA3108 菌株代表一个新种的假说,我们建议将其命名为 sp. nov。模式菌株为 CBA3108(=KACC 22889=JCM 35966)。