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sp. nov.,分离自马粪便。

sp. nov., isolated from horse faeces.

机构信息

Kimchi Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.

Department of Life Science, Chosun University, Gwangju 61452, Republic of Korea.

出版信息

Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006099.

Abstract

Strain CBA3108 is a Gram-positive, non-spore-forming, obligately anaerobic bacterium isolated from horse faecal samples obtained in Jeju Island, Republic of Korea. The cells of CBA3108 are non-motile short rods that have been assessed as catalase-positive and oxidase-negative. Growth of the strain occurs under the following conditions: 25-45 °C (optimum, 35 °C); pH 6-9 (optimum, pH 6); and in the presence of 0-6 % (w/v) NaCl (optimum, 2%). Major fatty acids in the strain include C iso and C iso DMA, while major polar lipids include phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. Based on phylogenetic analysis using 16S rRNA gene sequences, strain CBA3108 forms a phyletic lineage distinct from other closely related species within the genus . It was found to be most closely related to ATCC 25577 (98.27 % 16S rRNA gene sequence similarity) and other strains within the genus (≤98.0 %). The genomic DNA G+C content of strain CBA3108 was 63.2 mol%. The DNA-DNA hybridization values of strain CBA3108 with ATCC 25577, WCA-380-WT-3A and subsp. DSM 1897 were 33.6, 21.7 and 22.7 %, respectively. Its phenotypic, chemotaxonomic and molecular properties support the hypothesis that strain CBA3108 represents a novel species in the genus , for which we propose the name sp. nov. The type strain is CBA3108 (=KACC 22889=JCM 35966).

摘要

CBA3108 菌株是一株从韩国济州岛马粪便样本中分离得到的革兰氏阳性、非孢子形成、专性厌氧菌。CBA3108 细胞为短杆状,无动力,被鉴定为过氧化氢酶阳性和氧化酶阴性。该菌株的生长条件为:25-45℃(最适温度 35℃);pH 值 6-9(最适 pH 值 6);并且在 0-6%(w/v)NaCl(最适 2%)存在的情况下。该菌株的主要脂肪酸包括 Ciso 和 CisoDMA,而主要极性脂包括磷脂酰甘油、双磷脂酰甘油和磷脂酰胆碱。基于 16S rRNA 基因序列的系统发育分析,CBA3108 菌株与属内其他密切相关的种形成一个独特的进化枝。它与 ATCC 25577(98.27%的 16S rRNA 基因序列相似性)和属内的其他菌株(≤98.0%)最为密切相关。CBA3108 菌株的基因组 DNA G+C 含量为 63.2mol%。CBA3108 菌株与 ATCC 25577、WCA-380-WT-3A 和 subsp. DSM 1897 的 DNA-DNA 杂交值分别为 33.6%、21.7%和 22.7%。其表型、化学分类和分子特性支持了 CBA3108 菌株代表一个新种的假说,我们建议将其命名为 sp. nov。模式菌株为 CBA3108(=KACC 22889=JCM 35966)。

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