Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
School of Bioengineering, State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, PR China.
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005818.
A Gram-stain-positive, spore-forming and facultative aerobic bacterium, designated C11, was isolated from mountain soil collected in the Republic of Korea. The cells were motile rods with peritrichous flagella, and positive for catalase and oxidase activities. Strain C11 grew at 15-45 °C (optimum, 30-37 °C) and pH 6.0-8.0 (optimum, pH 6.0) and in the presence of 0-1 % (w/v) NaCl (optimum, 0.5 %). Strain C11 contained menaquinone-7 as the sole isoprenoid quinone and iso-C, iso-C and anteiso-C as the major fatty acids. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The G+C content of the genomic DNA was 38.8 mol%. Strain C11 was most closely related to IDA1967 and CV53, with 98.0 and 97.7 %, 71.7 and 69.9 %, and 20.1 and 20.3 % 16S rRNA gene sequence similarity, average nucleotide identity, and digital DNA-DNA hybridization values, respectively. Phylogenetic analyses based on 16S rRNA gene and genome sequences showed that strain C11 was included in a phyletic lineage with members of the genus but was distinct from members of the genus . Phenotypic, chemotaxonomic and molecular properties suggested that strain C11 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C11 (=KACC 21661=JCM 33943).
一株革兰氏阳性、产芽孢、兼性需氧细菌，被命名为 C11，从韩国采集的山地土壤中分离得到。细胞为周生鞭毛的能动杆状菌，过氧化氢酶和氧化酶活性均为阳性。C11 菌株在 15-45°C（最佳温度为 30-37°C）和 pH 6.0-8.0（最佳 pH 为 6.0）以及 0-1%（w/v）NaCl（最佳为 0.5%）的条件下生长。C11 菌株含有menaquinone-7 作为唯一的异戊二烯醌，iso-C、iso-C 和 anteiso-C 作为主要脂肪酸。二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺是主要的极性脂。基因组 DNA 的 G+C 含量为 38.8mol%。C11 菌株与 IDA1967 和 CV53 最为密切相关，16S rRNA 基因序列相似度分别为 98.0%和 97.7%，71.7%和 69.9%，20.1%和 20.3%；平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 71.7%和 69.9%，20.1%和 20.3%。基于 16S rRNA 基因和全基因组序列的系统发育分析表明，C11 菌株属于 but 属的一个分支，与 but 属的成员密切相关，但与 but 属的成员不同。表型、化学分类学和分子特性表明，C11 菌株代表 but 属的一个新种，提议命名为 but sp. nov.。模式菌株为 C11（=KACC 21661=JCM 33943）。
