Rahimi Kaveh, Riyahi Moslem, Sajedianfard Javad, Nazifi Saeed
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz.
Departments of Basic Sciences.
Ann Med Surg (Lond). 2023 Sep 20;85(11):5454-5458. doi: 10.1097/MS9.0000000000001252. eCollection 2023 Nov.
Therapeutic strategies with calcitonin gene-related peptide (CGRP) or its receptor have been investigated, but there are few studies regarding the possible harmful effects of CGRP in other body organs.
This study aimed to investigate the effect of intracerebroventricular (ICV) injection of CGRP on sex hormones and sperm quality in rats.
Twelve male rats were divided into two groups (=6 per group). The first group (control) rats were injected with 5 µl artificial cerebrospinal fluid intra-ICV; the second group rats, 5 µl (1.5 nmol) CGRP. The levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone were measured. Epididymal sperms were used to determine the sperm parameters.
The levels of testosterone, LH and FSH in CGRP group was significantly lower than in artificial cerebrospinal fluid (ACSF) group (<0.05). The concentration and motility of sperm in CGRP group was significantly lower than in ACSF group (<0.05). In CGRP group live spermatozoa and intact acrosome significantly reduced compared to the ACSF group (<0.05). In addition, in CGRP group dead spermatozoa and lose acrosome significantly increased compared to the ACSF group (<0.05).
ICV injection of CGRP may reduce sperm quality, probably through induction of an imbalance in FSH and LH production as well as testosterone.
已对降钙素基因相关肽（CGRP）或其受体的治疗策略进行了研究，但关于CGRP对其他身体器官可能产生的有害影响的研究较少。
本研究旨在探讨脑室内（ICV）注射CGRP对大鼠性激素和精子质量的影响。
将12只雄性大鼠分为两组（每组 = 6只）。第一组（对照组）大鼠经ICV注射5 μl人工脑脊液；第二组大鼠经ICV注射5 μl（1.5 nmol）CGRP。测量黄体生成素（LH）、卵泡刺激素（FSH）和睾酮水平。用附睾精子测定精子参数。
CGRP组的睾酮、LH和FSH水平显著低于人工脑脊液（ACSF）组（<0.05）。CGRP组的精子浓度和活力显著低于ACSF组（<0.05）。与ACSF组相比，CGRP组的活精子和完整顶体显著减少（<0.05）。此外，与ACSF组相比，CGRP组的死精子和顶体缺失显著增加（<0.05）。
ICV注射CGRP可能会降低精子质量，可能是通过诱导FSH、LH分泌以及睾酮产生失衡来实现的。
