Negri D J, Southwick P L, Brown W E
Biochim Biophys Acta. 1979 Jul 25;579(1):31-9. doi: 10.1016/0005-2795(79)90084-9.
4-Nitro-1-cyclohexyl-3-ethoxy-2-oxo-3-pyrroline reacts with both amino and sulfhydryl groups. The instability of the product with sulfhydryl groups makes the reagent a useful amino-group specific protein reagent. The advantages of this compound include (1) rapid reaction with protein (less than 15 min at pH 9), (2) EASE OF REVERSAL UNDER MILDLY ALKALINE CONDITIONS (PH larger than or equal to 8) with formation of a water-soluble by-product (lambdamax = 363 nm), and (3) ease of quantitation utilizing the high extinction coefficients of the amino derivative (lambdamax = 383 and 397 nm, epsilon397 = 20 200 M-1 . cm-1) and the reversal by-product (lambdamax = 363 nm, epsilon = 16 300 M-1 . cm-1). With these characteristics and the stability of the amino derivative under physiological conditions (t1/2 for reversal = 167 h at pH 7.0 and room temperature), nitrocyclohexylethoxyoxopyrroline can be a useful reagent in a wide variety of protein sequencing and structure studies.
4-硝基-1-环己基-3-乙氧基-2-氧代-3-吡咯啉能与氨基和巯基发生反应。与巯基反应产物的不稳定性使得该试剂成为一种有用的氨基特异性蛋白质试剂。该化合物的优点包括：（1）与蛋白质反应迅速（在pH 9时少于15分钟）；（2）在轻度碱性条件下（pH大于或等于8）易于逆转，形成水溶性副产物（最大吸收波长=363 nm）；（3）利用氨基衍生物（最大吸收波长=383和397 nm，ε397 = 20200 M-1·cm-1）和逆转副产物（最大吸收波长=363 nm，ε = 16300 M-1·cm-1）的高消光系数易于定量。具有这些特性以及氨基衍生物在生理条件下的稳定性（在pH 7.0和室温下逆转的半衰期=167小时），硝基环己基乙氧基氧代吡咯啉在各种蛋白质测序和结构研究中可能是一种有用的试剂。
