蛋白质和肽中氨基的测定。
The measurement of amino groups in proteins and peptides.
作者信息
Fields R
出版信息
Biochem J. 1971 Sep;124(3):581-90. doi: 10.1042/bj1240581.
Abstract
A technique is examined for determining amino groups with 2,4,6-trinitrobenzenesulphonic acid, in which the extinction at 420nm of sulphite complexes of the trinitrophenylated amino groups is measured. The sensitivity of the method is 5-200nmol of amino group. The method is especially suitable for checking the extent of blocking or unblocking of amino groups in proteins and peptides, owing to the short time required for reaction (5min at room temperature). The reaction of the reagent with thiol groups has been studied and was found to proceed 30-50 times faster than with in-amino groups of model compounds. The in(420) of a trinitrophenylated thiol group was found to be 2250m(-1).cm(-1). The reaction with several amino acids, peptides and proteins is presented. The in(420) of a typical alpha-amino group was found to be 22000m(-1).cm(-1) and that of an in-amino group, 19200m(-1).cm(-1). Difficulties inherent in the analysis of constituent amino group reactions in proteins are discussed.
摘要
研究了一种用2,4,6-三硝基苯磺酸测定氨基的技术，该技术通过测量三硝基苯基化氨基的亚硫酸盐络合物在420nm处的吸光度来进行。该方法的灵敏度为5-200nmol氨基。由于反应所需时间短（室温下5分钟），该方法特别适用于检测蛋白质和肽中氨基的封闭或去封闭程度。研究了该试剂与巯基的反应，发现其反应速度比与模型化合物的内氨基反应快30-50倍。发现三硝基苯基化巯基的ε(420)为2250m⁻¹·cm⁻¹。给出了该试剂与几种氨基酸、肽和蛋白质的反应情况。发现典型α-氨基的ε(420)为22000m⁻¹·cm⁻¹，内氨基的ε(420)为19200m⁻¹·cm⁻¹。讨论了蛋白质中组成氨基反应分析中固有的困难。
相似文献
1
The measurement of amino groups in proteins and peptides.蛋白质和肽中氨基的测定。
Biochem J. 1971 Sep;124(3):581-90. doi: 10.1042/bj1240581.
2
The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins.2,4,6-三硝基苯磺酸与氨基酸、肽和蛋白质的反应。
Biochem J. 1968 Jul;108(3):383-91. doi: 10.1042/bj1080383.
3
Effect of methylation on susceptibility of protein to proteolytic enzymes.甲基化对蛋白质对蛋白水解酶敏感性的影响。
Biochemistry. 1972 Jul 4;11(14):2589-93. doi: 10.1021/bi00764a006.
4
A new arylating agent, 2-carboxy-4,6-dinitrochlorobenzene. Reaction with model compounds and bovine pancreatic ribonuclease.一种新型芳基化试剂，2-羧基-4,6-二硝基氯苯。与模型化合物和牛胰核糖核酸酶的反应。
Int J Pept Protein Res. 1979;14(3):199-212. doi: 10.1111/j.1399-3011.1979.tb01926.x.
5
Restricted reactivity of the epsilon-amino groups of tobacco mosaic virus protein toward trinitrobenzenesulfonic acid.烟草花叶病毒蛋白质的ε-氨基对三硝基苯磺酸的反应活性受限。
Biochemistry. 1969 Sep;8(9):3597-603. doi: 10.1021/bi00837a016.
6
States of amino acid residues in proteins. XVI. Naphthoquinones with two sulfonic groups as a reagent for discrimation of amino groups in proteins.蛋白质中氨基酸残基的状态。十六。含两个磺酸基的萘醌作为区分蛋白质中氨基的试剂。
J Biochem. 1968 Oct;64(4):507-14. doi: 10.1093/oxfordjournals.jbchem.a128923.
7
Reactions of 2,4,6-trinitrobenzenesulfonate ion with amines and hydroxide ion.2,4,6-三硝基苯磺酸根离子与胺及氢氧根离子的反应。
Biochemistry. 1972 Sep 12;11(19):3564-71. doi: 10.1021/bi00769a011.
8
A kinetic study of the reactions of amino acids and peptides with trinitrobenzenesulfonic acid.氨基酸和肽与三硝基苯磺酸反应的动力学研究。
Biochemistry. 1966 Aug;5(8):2570-4. doi: 10.1021/bi00872a013.
9
Reactivity of amino groups in proteins.蛋白质中氨基的反应活性。
Biochim Biophys Acta. 1970 Jan 20;200(1):1-8. doi: 10.1016/0005-2795(70)90036-x.
10
Study of the dansylation reaction of amino acids, peptides and proteins.氨基酸、肽和蛋白质的丹磺酰化反应研究。
Eur J Biochem. 1969 Feb;7(4):463-70. doi: 10.1111/j.1432-1033.1969.tb19632.x.
引用本文的文献
1
Rapid formation of -(carboxymethyl)lysine (CML) from ribose depends on glyoxal production by oxidation.核糖快速形成β-(羧甲基)赖氨酸（CML）取决于氧化产生乙二醛。
RSC Chem Biol. 2024 Sep 18;5(11):1140-6. doi: 10.1039/d4cb00183d.
2
Hydrolyzed collagen: Exploring its applications in the food and beverage industries and assessing its impact on human health - A comprehensive review.水解胶原蛋白：探索其在食品和饮料行业中的应用并评估其对人体健康的影响——全面综述
Heliyon. 2024 Aug 16;10(16):e36433. doi: 10.1016/j.heliyon.2024.e36433. eCollection 2024 Aug 30.
3
An O-Specific Polysaccharide/Tetanus Toxoid Conjugate Vaccine Induces Protection in Guinea Pigs against Virulent Challenge with .一种O-特异性多糖/破伤风类毒素结合疫苗可诱导豚鼠对……的强毒株攻击产生保护作用。
Vaccines (Basel). 2022 Aug 25;10(9):1393. doi: 10.3390/vaccines10091393.
4
An overview of chemo- and site-selectivity aspects in the chemical conjugation of proteins.蛋白质化学偶联中化学选择性和位点选择性方面的概述。
R Soc Open Sci. 2022 Jan 26;9(1):211563. doi: 10.1098/rsos.211563. eCollection 2022 Jan.
5
Citraconylation and maleylation on the catalytic and thermodynamic properties of raw starch saccharifying amylase from .柠康酰化和马来酰化对来自[具体来源未给出]的生淀粉糖化淀粉酶催化和热力学性质的影响
Heliyon. 2020 Jul 3;6(7):e04351. doi: 10.1016/j.heliyon.2020.e04351. eCollection 2020 Jul.
6
Conformational changes of a chemically modified HRP: formation of a molten globule like structure at pH 5.化学修饰辣根过氧化物酶的构象变化：在pH 5时形成类似熔球态的结构
EXCLI J. 2014 May 27;13:611-22. eCollection 2014.
7
Chemical modifications of laccase from white-rot basidiomycete Cerrena unicolor.白腐真菌彩绒革盖菌漆酶的化学修饰。
Appl Biochem Biotechnol. 2012 Dec;168(7):1989-2003. doi: 10.1007/s12010-012-9912-4. Epub 2012 Oct 24.
8
Changing the enzyme reaction rate in magnetic nanosuspensions by a non-heating magnetic field.通过非加热磁场改变磁纳米悬浮液中的酶反应速率。
Angew Chem Int Ed Engl. 2012 Nov 26;51(48):12016-9. doi: 10.1002/anie.201205905. Epub 2012 Oct 18.
9
Glutaraldehyde erythropoietin protects kidney in ischaemia/reperfusion injury without increasing red blood cell production.戊二醛促红细胞生成素可防止肾缺血/再灌注损伤，而不增加红细胞生成。
Br J Pharmacol. 2013 Jan;168(1):189-99. doi: 10.1111/j.1476-5381.2012.02123.x.
10
Characterization of valvular interstitial cell function in three dimensional matrix metalloproteinase degradable PEG hydrogels.三维基质金属蛋白酶可降解聚乙二醇水凝胶中瓣膜间质细胞功能的表征
Biomaterials. 2009 Dec;30(34):6593-603. doi: 10.1016/j.biomaterials.2009.08.031. Epub 2009 Sep 10.
本文引用的文献
1
REACTION BETWEEN SULFHYDRYL COMPOUNDS AND 2,4,6-TRINITROBENZENE-1-SULFONIC ACID.巯基化合物与2,4,6-三硝基苯-1-磺酸之间的反应
J Biochem. 1964 May;55:553-61.
2
A colour reaction of vitamin E with vitamin K and cysteine.维生素E与维生素K及半胱氨酸的显色反应。
Nature. 1958 Jan 4;181(4601):49. doi: 10.1038/181049a0.
3
Some spectrophotometric and polarimetric experiments with ribonuclease.一些用核糖核酸酶进行的分光光度法和旋光测定实验。
Biochim Biophys Acta. 1957 May;24(2):229-35. doi: 10.1016/0006-3002(57)90186-5.
4
A kinetic study of the reactions on some disulphides with sodium sulphite.某些二硫化物与亚硫酸钠反应的动力学研究。
Biochem J. 1955 Jul;60(3):496-506. doi: 10.1042/bj0600496.
5
Tertiary structure of ribonuclease.核糖核酸酶的三级结构。
Nature. 1967 Mar 4;213(5079):862-5. doi: 10.1038/213862a0.
6
A kinetic study of the reactions of amino acids and peptides with trinitrobenzenesulfonic acid.氨基酸和肽与三硝基苯磺酸反应的动力学研究。
Biochemistry. 1966 Aug;5(8):2570-4. doi: 10.1021/bi00872a013.
7
The reaction of 2,4,6-trinitrobenzenesulphonic acid with amino acids, Peptides and proteins.2,4,6-三硝基苯磺酸与氨基酸、肽和蛋白质的反应。
Biochem J. 1968 Jul;108(3):383-91. doi: 10.1042/bj1080383.
8
The reaction of aldolase with 2-methylmaleic anhydride.醛缩酶与2-甲基马来酸酐的反应。
Biochem J. 1970 Mar;116(5):843-9. doi: 10.1042/bj1160843.
9
The reactivity of thiol groups and the subunit structure of aldolase.硫醇基团的反应性与醛缩酶的亚基结构
Biochem J. 1970 Apr;117(2):291-8. doi: 10.1042/bj1170291.
10
Purification of ribonuclease T 1 by diethylaminoethylcellulose chromatography.用二乙氨基乙基纤维素色谱法纯化核糖核酸酶T1
Biochem J. 1971 Feb;121(4):591-6. doi: 10.1042/bj1210591.
Zotero 插件