Machado Sinara Silva Neves, Silva Jania Betânia Alves da, Nascimento Renata Quartieri, Lemos Paulo Vitor França, Assis Denílson de Jesus, Marcelino Henrique Rodrigues, Ferreira Ederlan de Souza, Cardoso Lucas Guimarães, Pereira Juraci Duarte, Santana Jamille Santos, Silva Maria Luiza Andrade da, Souza Carolina Oliveira de
Graduate Program in Food Science, Faculty of Pharmacy, Federal University of Bahia, Salvador, BA, Brazil.
Center for Exact and Technological Sciences, Faculty of Mechanical Engineering, Federal University of Recôncavo da Bahia, Cruz das Almas, BA, Brazil; Graduate Program in Chemical Engineering, Polytechnic School, Federal University of Bahia, Salvador, BA, Brazil.
Int J Biol Macromol. 2024 Jan;254(Pt 3):127773. doi: 10.1016/j.ijbiomac.2023.127773. Epub 2023 Nov 3.
This work aimed to obtain and characterize chitin and chitosan extracted from the rearing residues of Tenebrio molitor, Zophobas morio, and Blaptica dubia insects in different growth stages in the same rearing cycles chitin and chitosan yielded 11.21 %-20.89 % and 6.26 %-7.07 %, respectively. The deacetylation degrees of chitosan ranged from 75.75 %-89.21 %, and the solubilities from 69.88 %-94.39 %. Infrared spectroscopy corroborated the acquisition of chitin and chitosan and can be used as a semi-quantitative technique for determining the degree of chitosan deacetylation. The X-ray diffraction profiles revealed the presence of α-chitin, and the relative crystalline indices ranged from 65.9 %-89.2 %. Typical TG profiles with two thermal events are observed for chitin and chitosan samples with different residue contents from the extraction procedure. The chitosan solutions exhibited pseudoplastic behavior, with apparent viscosities ranging from 195.96 to 249.86 mPa.s. The characterization results of the biopolymers extracted from insect residues were similar to those obtained from conventional sources. The growth stage influenced the chitin yield and crystallinity index. The results of this study reinforce the feasibility of using alternative sources of chitin and chitosan, providing the use of waste from insect farms and contributing to sustainability and a circular economy.
这项工作旨在从黄粉虫、大王花金龟和杜比亚蟑螂在相同饲养周期不同生长阶段的饲养残渣中提取几丁质和壳聚糖并对其进行表征。几丁质和壳聚糖的产率分别为11.21% - 20.89%和6.26% - 7.07%。壳聚糖的脱乙酰度在75.75% - 89.21%之间，溶解度在69.88% - 94.39%之间。红外光谱证实了几丁质和壳聚糖的获得，并且可用作测定壳聚糖脱乙酰度的半定量技术。X射线衍射图谱显示存在α-几丁质，相对结晶指数在65.9% - 89.2%之间。对于来自提取过程中具有不同残渣含量的几丁质和壳聚糖样品，观察到具有两个热事件的典型热重曲线。壳聚糖溶液表现出假塑性行为，表观粘度范围为195.96至249.86 mPa·s。从昆虫残渣中提取的生物聚合物的表征结果与从传统来源获得的结果相似。生长阶段影响几丁质产率和结晶度指数。本研究结果强化了使用几丁质和壳聚糖替代来源的可行性，为昆虫养殖场废物的利用提供了支持，并有助于可持续性和循环经济。
