Shaala A Y, Dhaliwal H S, Bishop S, Ling N R
J Immunol Methods. 1979;27(2):175-87. doi: 10.1016/0022-1759(79)90263-1.
Procion-dyed yeasts which have been incubated in fresh serum and washed are readily ingested by human blood monocytes and tumour macrophages during a 30 min incubation period. Uptake is enhanced by centrifugation. Intracellular yeasts can be readily distinquished from extracellular by their much slower uptake of toluidine blue. Yeast ingestion is a much more reliable test for blood monocytes than the latex bead test and it is easier to read. The ingestion test may be combined with a rosette test for surface immunoglobulins (SmIg). Since the yeasts take up immunoglobulins from human serum during the complement-coating stage it is necessary, in a combined ingestion-SmIg test, to use fresh serum from another species (sheep) for opsonisation of the yeasts. A technique is described for reducing the number of immunoglobulin-bearing monocytes to a low level with a combined ingestion-SmIg rosette technique to detect residual immunoglobulin-bearing phagocytes.
经在新鲜血清中孵育并洗涤的普施安染色酵母，在30分钟的孵育期内很容易被人血单核细胞和肿瘤巨噬细胞摄取。通过离心可增强摄取。细胞内酵母因其摄取甲苯胺蓝的速度慢得多，所以很容易与细胞外酵母区分开来。酵母摄取试验对血单核细胞来说比乳胶珠试验更可靠，且更容易判读。摄取试验可与表面免疫球蛋白（SmIg）的玫瑰花结试验相结合。由于酵母在补体包被阶段会从人血清中摄取免疫球蛋白，因此在联合摄取 - SmIg试验中，有必要使用来自另一个物种（绵羊）的新鲜血清对酵母进行调理。本文描述了一种用联合摄取 - SmIg玫瑰花结技术将携带免疫球蛋白的单核细胞数量降低到低水平的方法，以检测残留的携带免疫球蛋白的吞噬细胞。
