Elsayed A, Richart R M, Crum C P
Gynecol Oncol. 1987 Jan;26(1):25-34. doi: 10.1016/0090-8258(87)90068-0.
Involucrin is a keratinocyte envelope protein precursor which is synthesized at an early stage of differentiation in normal squamous epithelium. Recent studies suggest that this protein may be a marker for neoplastic epithelium. To address this issue, we analyzed involucrin expression in 105 biopsies containing 119 areas of normal, condylomatous, and neoplastic epithelium. Overall, 88, 75, and 55% of condylomata, well-differentiated CIN, and poorly differentiated CIN (carcinoma in situ) contained positive staining for involucrin. Excluding lesions with severe inflammation, 100, 88, and 55% of these lesions, respectively, were positive. Staining patterns in neoplastic lesions differed from those in the normal epithelium and condylomata; the staining in CIN tended to be focal, and intensity of staining varied widely from cell to cell in all layers of the epithelium. In high grade CIN, staining correlated with increases in cell size and cytoplasmic differentiation. These studies suggest that involucrin will not differentiate between lesions of low versus high risk for progressing to invasive carcinoma. However, the patterns of involucrin expression confirm the marked differences in patterns of cellular differentiation between classical condylomata and CIN.
兜甲蛋白是一种角质形成细胞包膜蛋白前体，在正常鳞状上皮分化的早期阶段合成。最近的研究表明，这种蛋白可能是肿瘤上皮的标志物。为解决这一问题，我们分析了105份活检标本中119个区域的正常、湿疣样和肿瘤上皮的兜甲蛋白表达情况。总体而言，88%、75%和55%的湿疣、高分化CIN和低分化CIN（原位癌）的兜甲蛋白染色呈阳性。排除伴有严重炎症的病变后，这些病变分别有100%、88%和55%呈阳性。肿瘤病变的染色模式与正常上皮和湿疣不同；CIN中的染色往往呈局灶性，且上皮各层细胞间的染色强度差异很大。在高级别CIN中，染色与细胞大小增加和细胞质分化相关。这些研究表明，兜甲蛋白无法区分进展为浸润性癌的低风险和高风险病变。然而，兜甲蛋白的表达模式证实了经典湿疣和CIN在细胞分化模式上的显著差异。
