Lopes Thainara, Fidelis Carlos E, Silva Amanda T F, Mota Rinaldo A, Rall Vera L M, Dos Santos Marcos V, Gonçalves Juliano L
School of Animal Science and Food Engineering, University of São Paulo, Pirassununga 13635-900, Brazil.
Qualileite, Department of Animal Nutrition and Production, School of Veterinary Medicine and Animal Sciences, University of São Paulo, Pirassununga 13635-900, Brazil.
J Appl Microbiol. 2023 Nov 1;134(11). doi: 10.1093/jambio/lxad249.
This study aimed to evaluate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial subtyping for the rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis.
A total of 229 S. aureus isolates were obtained from milk samples collected from cows with subclinical mastitis using microbiological culture. Staphylococcus aureus isolates were also submitted to PCR analysis targeting the mecA and mecC genes, which are indicative of methicillin resistance. Confirmation of the species was achieved through MALDI-TOF MS analysis. To analyze antimicrobial resistance patterns, the MALDI BioTyper Compass Explorer and ClinProTools Bruker software were employed, and dendrograms were generated using Bionumerics software.
MALDI-TOF MS successfully identified S. aureus at the species level, but no methicillin resistance was observed. Moreover, spectral typing displayed limited similarity when compared to pulsed-field gel electrophoresis (PFGE).
本研究旨在评估基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）细菌亚型分析，用于从亚临床型牛乳腺炎中快速检测金黄色葡萄球菌的生物标志物。
通过微生物培养从患有亚临床乳腺炎的奶牛采集的牛奶样本中，共获得229株金黄色葡萄球菌分离株。金黄色葡萄球菌分离株还进行了针对mecA和mecC基因的PCR分析，这两个基因指示耐甲氧西林情况。通过MALDI-TOF MS分析实现了菌种确认。为分析抗菌药物耐药模式，使用了MALDI BioTyper Compass Explorer和ClinProTools Bruker软件，并使用Bionumerics软件生成树形图。
MALDI-TOF MS成功在菌种水平鉴定出金黄色葡萄球菌，但未观察到耐甲氧西林情况。此外，与脉冲场凝胶电泳（PFGE）相比，光谱分型显示出有限的相似性。
