Wanecka Anna, Król Jarosław, Twardoń Jan, Mrowiec Jacek, Korzeniowska-Kowal Agnieszka, Wzorek Anna
Departments of Pathology (Wanecka, Król), Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Reproduction and Clinic of Farm Animals (Twardoń, Mrowiec), Faculty of Veterinary Medicine, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
J Vet Diagn Invest. 2019 Jul;31(4):523-530. doi: 10.1177/1040638719845423. Epub 2019 Apr 22.
We compared the effectiveness of various methods for the identification of spp. other than S. isolated from intramammary infections of cows on 3 dairy farms in Lower Silesia, Poland. A total of 131 isolates belonging to 18 species were identified by sequence analysis of the 16S rRNA and genes, as well using a commercial identification system (ID 32 STAPH; bioMérieux) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS; Bruker Daltonics). Sequencing of the 16S rRNA gene was found to have low discriminatory value because only 43% of isolates were recognized unequivocally. Much better results were obtained with the gene (all isolates were correctly identified at the species level). However, some of these isolates achieved a low similarity level (<97%) and required a confirmatory test (sequencing of the gene). The performance of ID 32 STAPH was poor. Regardless of the probability level used (80% or 90%), the commercial system obtained identification rates <40%. Using MALDI-TOF MS and the commercial Bruker database, 67% of isolates were identified correctly with scores ≥2.0 (acceptable species-level identification) but this number increased to 97% after the database was expanded. The definitive identification of spp. other than causing intramammary infections in cattle often requires a combination of different procedures, and the existing databases should be updated.
我们比较了多种方法在鉴定从波兰下西里西亚3个奶牛场患有乳房内感染的奶牛中分离出的除金黄色葡萄球菌外的其他葡萄球菌属物种方面的有效性。通过对16S rRNA和[未提及的基因名称]基因进行序列分析，以及使用商业鉴定系统（ID 32 STAPH；bioMérieux）和基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS；Bruker Daltonics），共鉴定出属于18个物种的131株分离株。发现16S rRNA基因测序的鉴别价值较低，因为只有43%的分离株能被明确识别。[未提及的基因名称]基因测序获得了更好的结果（所有分离株在物种水平上均被正确鉴定）。然而，其中一些分离株的相似度水平较低（<97%），需要进行确证试验（[未提及的基因名称]基因测序）。ID 32 STAPH的性能较差。无论使用的概率水平是80%还是90%，该商业系统的鉴定率均<40%。使用MALDI-TOF MS和商业布鲁克数据库，67%的分离株在得分≥2.0时被正确鉴定（可接受的物种水平鉴定），但在数据库扩展后，这一数字增加到了97%。对引起牛乳房内感染的除金黄色葡萄球菌外的其他葡萄球菌属物种进行最终鉴定通常需要结合不同的程序，并且现有数据库应更新。
