Pharmacy Academy of Xinjiang Medical University, Urumqi, 830054, People's Republic of China.
Xinjiang Key Laboratory of Natural Medicine Active Components and Drug Release Technology, Urumqi, 830054, People's Republic of China.
Nanotechnology. 2023 Nov 29;35(7). doi: 10.1088/1361-6528/ad0a4a.
In this study, a surface-enhanced Raman spectroscopy (SERS) magnetic sensor is established based on SERS principle and magnetic separation technology, and a highly sensitive, simple and fast method for quantitative detection of neutralizing antibodies (nABs) and specific IgG of SARS-CoV-2 in plasma is established combined with immunoassay. Two kinds of Raman nanospheres (RNPs) with different characteristic Raman shifts are used as signal sources and coupled to ACE2 and anti-IgG (FC) antibodies respectively, and magnetic beads are coupled to RBD. The competitive relationship between ACE2 and nABs, the binding relationship between specific IgG and anti-IgG (FC) antibodies are determined. The results show that the concentrations of nABs and specific IgG in the range of 10-2000 ng mlare well correlated with SERS response intensity, and the recoveries are both between 90%-110%, with good precision. Bilirubin and common anticoagulants have no interference on the detection results. This method is accurate, reliable, sensitive and does not require complex pre-treatment, and is expected to be used for simultaneous detection of nABs and specific IgG in plasma of SARS-CoV-2. It has guiding significance for the development and evaluation of vaccines and the formulation of individualized vaccination schedule.
本研究基于表面增强拉曼光谱（SERS）原理和磁分离技术，建立了一种 SERS 磁传感器，并结合免疫测定法，建立了一种灵敏、简单、快速的定量检测血浆中中和抗体（nABs）和 SARS-CoV-2 特异性 IgG 的方法。两种具有不同特征拉曼位移的拉曼纳米球（RNP）分别作为信号源，与 ACE2 和抗 IgG（FC）抗体偶联，磁珠与 RBD 偶联。确定了 ACE2 与 nABs 之间的竞争关系，以及特异性 IgG 与抗 IgG（FC）抗体之间的结合关系。结果表明，nABs 和特异性 IgG 的浓度在 10-2000ngml 范围内与 SERS 响应强度呈良好的相关性，回收率均在 90%-110%之间，具有良好的精密度。胆红素和常见的抗凝剂对检测结果没有干扰。该方法准确、可靠、灵敏，不需要复杂的预处理，有望用于同时检测 SARS-CoV-2 血浆中的 nABs 和特异性 IgG。对疫苗的开发和评价以及个体化免疫接种方案的制定具有指导意义。
