Wellcome Sanger Institute, Cambridgeshire, CB10 1SA, Hinxton, UK.
School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, G12 8TA, UK.
Sci Data. 2023 Nov 7;10(1):775. doi: 10.1038/s41597-023-02674-2.
The flatworm Schistosoma mansoni is an important but neglected pathogen that causes the disease schistosomiasis in millions of people worldwide. The parasite has a complex life cycle, undergoing sexual reproduction in a mammalian host and asexual replication in a snail host. Understanding the molecular mechanisms that the parasite uses to transition between hosts and develop into dimorphic reproductively competent adults may reveal new strategies for control. We present the first comprehensive transcriptomic analysis of S. mansoni, from eggs to sexually naïve worms. Focusing on eight life stages spanning free-living water-borne and parasitic stages from both intermediate and definitive hosts, we have generated deep RNA-seq data for five replicates per group for a total of 75 data sets. The data were produced using a single approach to increase the accuracy of stage-to-stage comparisons and made accessible via a user-friendly tool to visualise and explore gene expression ( https://lifecycle.schisto.xyz/ ). These data are valuable for understanding the biology and sex-specific development of schistosomes and the interpretation of complementary genomic and functional genetics studies.
曼氏血吸虫是一种重要但被忽视的病原体，它可导致全世界数百万人罹患血吸虫病。该寄生虫具有复杂的生活史，在哺乳动物宿主中进行有性繁殖，在蜗牛宿主中进行无性复制。了解寄生虫在宿主间转换并发育为二态性有性生殖能力成虫的分子机制，可能揭示新的控制策略。我们对曼氏血吸虫进行了首次全面的转录组分析，从卵到性未成熟的蠕虫。研究集中在 8 个生活阶段，涵盖了中间宿主和终末宿主的自由生活水生阶段和寄生阶段，每组有 5 个重复，总共产生了 75 个数据集。这些数据是使用单一方法生成的，以提高阶段间比较的准确性，并通过用户友好的工具（https://lifecycle.schisto.xyz/）使其易于访问，以可视化和探索基因表达。这些数据对于理解血吸虫的生物学和性别特异性发育以及解释互补的基因组和功能遗传学研究具有重要价值。
