Neto Brenno A D, Sorto Jenny E P, Lapis Alexandre A M, Machado Fabricio
Laboratory of Medicinal and Technological Chemistry, University of Brasília, Chemistry Institute (IQ-UnB), Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal 70910-900, Brazil.
Laboratory of Medicinal and Technological Chemistry, University of Brasília, Chemistry Institute (IQ-UnB), Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal 70910-900, Brazil; Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife 50670-901, PE, Brazil.
Methods. 2023 Dec;220:142-157. doi: 10.1016/j.ymeth.2023.11.001. Epub 2023 Nov 6.
This review aims to provide a comprehensive overview of recent advancements and applications of fluorescence imaging probes synthesized via MCRs (multicomponent reactions). These probes, also known as functional chromophores, belong to a currently investigated class of fluorophores that are presently being successfully applied in bioimaging experiments, especially in various living cell lineages. We describe some of the MCRs that have been employed in the synthesis of these probes and explore their applications in biological imaging, with an emphasis on cellular imaging. The review also discusses the challenges and future perspectives in the field, particularly considering the potential impact of MCR-based fluorescence imaging probes on advancing this field of research in the coming years. Considering that this area of research is relatively new and nearly a decade has passed since the first publication, this review also provides a historical perspective on this class of fluorophores, highlighting the pioneering works published between 2011 and 2016.
本综述旨在全面概述通过多组分反应（MCRs）合成的荧光成像探针的最新进展和应用。这些探针，也被称为功能发色团，属于目前正在研究的一类荧光团，目前已成功应用于生物成像实验，特别是在各种活细胞谱系中。我们描述了一些用于合成这些探针的多组分反应，并探讨了它们在生物成像中的应用，重点是细胞成像。本综述还讨论了该领域的挑战和未来前景，特别是考虑到基于多组分反应的荧光成像探针在未来几年对推动这一研究领域的潜在影响。鉴于该研究领域相对较新，自首次发表以来已过去近十年，本综述还提供了关于这类荧光团的历史视角，突出了2011年至2016年间发表的开创性工作。
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