Lahmy S, Salmon J M, Viallet P
J Histochem Cytochem. 1987 Feb;35(2):197-201. doi: 10.1177/35.2.3794313.
The purpose of this study was to determine if dibenzo(c,h)acridine could be used as a probe for the N-heterocyclic aromatic compound detoxification system. The determination was achieved by cell population studies (histograms) using microspectrofluorimetry on single living cells. The results show a similar metabolic distribution pattern between dibenzo(c,h)acridine and benzo(a)pyrene, whereas important differences appear between 6-aminochrysene and dibenzo(c,h)acridine, respectively amine aromatic and N-heterocyclic aromatic compounds. No differences were observed in the metabolism of benzo(a)pyrene in untreated cells and those treated with constant concentrations of dibenzo(c,h)acridine. However, inhibition of dibenzo(c,h)acridine metabolism was observed in the presence of benzo(a)pyrene, indicating that this compound should be a preferential substrate for a cell detoxification system.
本研究的目的是确定二苯并(c,h)吖啶是否可作为N-杂环芳香族化合物解毒系统的探针。该测定通过使用显微分光荧光测定法对单个活细胞进行细胞群体研究（直方图）来实现。结果显示二苯并(c,h)吖啶和苯并(a)芘之间存在相似的代谢分布模式，而6-氨基 Chrysene和二苯并(c,h)吖啶之间分别出现重要差异，前者为胺类芳香族化合物，后者为N-杂环芳香族化合物。在未处理的细胞和用恒定浓度的二苯并(c,h)吖啶处理的细胞中，未观察到苯并(a)芘代谢的差异。然而，在存在苯并(a)芘的情况下，观察到二苯并(c,h)吖啶代谢受到抑制，这表明该化合物应该是细胞解毒系统的优先底物。
