Lahmy S, Salmon J M, Viallet P
Toxicology. 1984 Feb;29(4):345-56. doi: 10.1016/0300-483x(84)90166-5.
By microspectrofluorimetry on single living cells (murine fibroblasts 3T3), we have obtained monoexponential decreases of fluorescence intensity for benzo[a]pyrene (B[a]P) and 6-aminochrysene (6a-chrysene) metabolism. These kinetics are characteristics of B[a]P and 6a-chrysene metabolism and histograms can be drawn from the rate constants. We have studied the influence of 6a-chrysene on B[alpha]P metabolism. Using different methods of incubation, it has been observed that the presence of 6a-chrysene leads to modifications of the histogram profiles during B[a]P metabolism. Polycyclic aromatic hydrocarbons (PAH) are used to induce B[a]P metabolism. Whatever the experimental conditions we never detected such a phenomenon with 6a-chrysene. On the contrary we have observed an inhibition of B[a]P metabolism by 6a-chrysene, which can reach 80% of the aryl hydrocarbon hydroxylase (AHH) activity when 6a-chrysene remains constant in the cells. Compared with the results previously observed "in vitro" which presented 50% mean inhibition) we show that inhibition acts in an all-or-nothing mechanism at the cellular level.
通过对单个活细胞（小鼠成纤维细胞3T3）进行显微分光荧光测定，我们获得了苯并[a]芘（B[a]P）和6-氨基芘（6a-芘）代谢过程中荧光强度的单指数下降。这些动力学是B[a]P和6a-芘代谢的特征，并且可以根据速率常数绘制直方图。我们研究了6a-芘对B[α]P代谢的影响。使用不同的孵育方法，已观察到6a-芘的存在会导致B[a]P代谢过程中直方图轮廓的改变。多环芳烃（PAH）用于诱导B[a]P代谢。无论实验条件如何，我们从未在6a-芘中检测到这种现象。相反，我们观察到6a-芘对B[a]P代谢有抑制作用，当细胞中6a-芘保持恒定时，这种抑制作用可达到芳烃羟化酶（AHH）活性的80%。与先前在“体外”观察到的结果（平均抑制率为50%）相比，我们表明这种抑制作用在细胞水平上以全或无机制起作用。
