Westgeest A A, Bons J C, Van den Brink H G, Aarden L A, Smeenk R J
J Immunol Methods. 1986 Dec 24;95(2):283-8. doi: 10.1016/0022-1759(86)90417-5.
To facilitate the use of Western blots for the detection of antibodies, we have developed an incubation apparatus. The use of this apparatus simplifies the incubation of blots with antisera, permits the testing of large numbers of sera and eliminates artefacts caused by the use of loose strips. The introduction of a pressure bag in the lower lid of the apparatus secures a steady pressure over the entire blot, a feature lacking in currently available commercial equipment. The detection of antinuclear antibodies is presented as an example of the use of this incubator.
为便于使用蛋白质免疫印迹法检测抗体，我们研发了一种孵育装置。该装置的使用简化了印迹与抗血清的孵育过程，能够检测大量血清，并消除了使用松散条带所导致的假象。在该装置的下盖中引入压力袋可确保在整个印迹上施加稳定的压力，这是目前市售设备所不具备的特性。本文以抗核抗体的检测为例展示了该孵育器的使用方法。
