Warlow R, Carrano J, Dawkins R
Diagn Immunol. 1984;2(3):154-60.
An enzyme linked immunosorbent assay (ELISA) has been developed to detect antinuclear antibodies to extractable nuclear antigens (ENA)--a rabbit thymus nuclear derivative. A second ELISA has also been developed (ENA RNA-ase ELISA) to analyse the relative contribution of the components of ENA (nucleoprotein and RNA antigens) to antibody binding as detected by the ELISA assays. Both ELISA assays provide a rapid screening method for antibodies to soluble, diffusable nuclear antigens that could otherwise go undetected by immunodiffusion or the indirect immunofluorescent method that uses unfixed frozen sections of rat liver. The considerable improvement that these ELISA assays offer in efficiency and both diagnostic and serological sensitivity over the traditional immunodiffusion method used for specificity characterisation of ENA autoantibodies is discussed.
已经开发出一种酶联免疫吸附测定（ELISA）来检测针对可提取核抗原（ENA）——一种兔胸腺核衍生物——的抗核抗体。还开发了第二种ELISA（ENA RNA酶ELISA），以分析ELISA检测中ENA成分（核蛋白和RNA抗原）对抗体结合的相对贡献。这两种ELISA测定都为可溶性、可扩散核抗原的抗体提供了一种快速筛选方法，否则这些抗体可能无法通过免疫扩散或使用大鼠肝脏未固定冰冻切片的间接免疫荧光法检测到。本文讨论了这些ELISA测定在效率以及诊断和血清学敏感性方面相对于用于ENA自身抗体特异性表征的传统免疫扩散方法所提供的显著改进。
