Nana Rodrigue Roman Dongang, Ngum Ngum Lesly, Makoge Valerie, Amvongo-Adja Nathalie, Hawadak Joseph, Singh Vineeta
Institute of Medical Research and Medicinal Plants studies (IMPM), P.O Box 13033 Yaoundé, Cameroon; Parasite and Host Biology Group, ICMR-National Institute of Malaria Research, New Delhi 110077, India..
Institute of Medical Research and Medicinal Plants studies (IMPM), P.O Box 13033 Yaoundé, Cameroon.
Diagn Microbiol Infect Dis. 2024 Jan;108(1):116103. doi: 10.1016/j.diagmicrobio.2023.116103. Epub 2023 Oct 17.
Malaria rapid diagnostic tests (mRDT) play a vital role in malaria control in endemic areas. In this study, histidine-rich protein (hrp) and lactate dehydrogenase (ldh) genes were genotyped in Plasmodium falciparum (Pf) and Plasmodium ovale (Po) spp. isolates. Deletions in P. falciparum hrp2/3 (pfhrp2/3) proteins and single nucleotide polymorphisms (SNPs) were analyzed. Twenty-four samples were analyzed for pfhrp2/3 gene deletions and 25 for SNPs in ldh gene (18 Pf and 7 Po spp.). Deletions in pfhrp2/3 genes were observed in 1.9% malaria positive isolates. The pfldh gene sequences showed one SNP at codon 272 (D272N) in 22.2% of samples while in Po spp., sequences were 100% similar to P. ovale curtisi but when compared to P. ovale wallikeri reference sequence, SNPs at positions 143 (P143S), 168 (K168N), 204 (V204I) were found. Findings suggest low prevalence in pfhrp2/3 genes and highlight the circulation of P. ovale curtisi in the studies areas.
疟疾快速诊断检测（mRDT）在疟疾流行地区的疟疾防控中发挥着至关重要的作用。在本研究中，对恶性疟原虫（Pf）和卵形疟原虫（Po）分离株中的富含组氨酸蛋白（hrp）和乳酸脱氢酶（ldh）基因进行了基因分型。分析了恶性疟原虫hrp2/3（pfhrp2/3）蛋白的缺失和单核苷酸多态性（SNP）。对24个样本进行了pfhrp2/3基因缺失分析，对25个样本进行了ldh基因SNP分析（18个Pf和7个Po分离株）。在1.9%的疟疾阳性分离株中观察到pfhrp2/3基因缺失。pfldh基因序列在22.2%的样本中第272位密码子处显示一个SNP（D272N），而在Po分离株中，序列与柯氏卵形疟原虫100%相似，但与沃氏卵形疟原虫参考序列相比，在第143位（P143S）、168位（K168N）、204位（V204I）发现了SNP。研究结果表明pfhrp2/3基因的流行率较低，并突出了研究区域内柯氏卵形疟原虫的传播情况。
