Hawadak Joseph, Foko Loick Pradel Kojom, Nana Rodrigue Roman Dongang, Singh Vineeta
ICMR-National Institute of Malaria Research (NIMR), Delhi,, 110077, India.
Institut de Recherches Médicales et D'Etudes des Plantes Médicinales (IMPM), Yaoundé, Cameroon.
Curr Microbiol. 2025 Feb 7;82(3):125. doi: 10.1007/s00284-025-04106-3.
Circumsporozoite protein (CSP) is the primary component of two malaria vaccines that have been validated by World Health Organisation. However, these vaccines do not include antigens from non-falciparum species, which should also be considered. The csp gene was amplified and sequenced in 14 Plasmodium ovale cuitisi (Poc) isolates from Cameroon. The C and N-terminal regions showed perfect conservation, while the central region showed repeated motifs of nine amino acids. Interestingly, in addition to nano-repeat motifs reported in the literature, a new motif (QSEGNPPAP) was observed in four samples, resulting in haplotype combinations that can affect the antigenicity of protein and therefore should be taken into account when designing a CSP-based vaccine against Poc malaria.
环子孢子蛋白（CSP）是两种已获世界卫生组织验证的疟疾疫苗的主要成分。然而，这些疫苗并不包含来自非恶性疟原虫物种的抗原，而这些抗原也应予以考虑。对来自喀麦隆的14株卵形疟原虫库伊蒂亚种（Poc）分离株的csp基因进行了扩增和测序。C端和N端区域显示出完美的保守性，而中央区域则显示出九个氨基酸的重复基序。有趣的是，除了文献中报道的纳米重复基序外，在四个样本中还观察到一个新的基序（QSEGNPPAP），这导致了单倍型组合，可能会影响蛋白质的抗原性，因此在设计针对Poc疟疾的基于CSP的疫苗时应予以考虑。
