转录因子，帽结合蛋白同型 C 调控参与昆虫对杀虫剂敏感性的 CYP450 基因的表达。
Transcription factors, cap 'n' collar isoform C regulates the expression of CYP450 genes involving in insecticides susceptibility in Locusta migratoria.
机构信息
Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; Shanxi Key Laboratory of Nucleic Acid Biopesticides, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, China.
Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China; Shanxi Key Laboratory of Nucleic Acid Biopesticides, China; Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, China.
出版信息
Pestic Biochem Physiol. 2023 Nov;196:105627. doi: 10.1016/j.pestbp.2023.105627. Epub 2023 Sep 22.
BACKGROUND
The cap 'n' collar (Cnc) belongs to the Basic Leucine Zipper (bZIP) transcription factor super family. Cap 'n' collar isoform C (CncC) is highly conserved in the animal kingdom. CncC contributes to the regulation of growth, development, and aging and takes part in the maintenance of homeostasis and the defense against endogenous and environmental stress. Insect CncC participates in the regulation of various kinds of stress-responsive genes and is involved in the development of insecticide resistance.
RESULTS
In this study, one full-length CncC sequence of Locusta migratoria was identified and characterized. Upon RNAi silencing of LmCncC, insecticide bioassays showed that LmCncC played an essential role in deltamethrin and imidacloprid susceptibility. To fully investigate the downstream genes regulated by LmCncC and further identify the LmCncC-regulated genes involved in deltamethrin and imidacloprid susceptibility, a comparative transcriptome was constructed. Thirty-five up-regulated genes and 73 down-regulated genes were screened from dsLmCncC-knockdown individuals. We selected 22 LmCncC-regulated genes and verified their gene expression levels using RT-qPCR. Finally, six LmCYP450 genes belonging to the CYP6 family were selected as candidate detoxification genes, and LmCYP6FD1 and LmCYP6FE1 were further validated as detoxification genes of insecticides via RNAi, insecticide bioassays, and metabolite identification.
CONCLUSIONS
Our data suggest that the locust CncC gene is associated with deltamethrin and imidacloprid susceptibility via the regulation of LmCYP6FD1 and LmCYP6FE1, respectively.
背景
帽领（Cnc）属于基本亮氨酸拉链（bZIP）转录因子超家族。帽领同工型 C（CncC）在动物界高度保守。CncC 有助于调节生长、发育和衰老，并参与维持体内平衡和防御内源性和环境应激。昆虫 CncC 参与调节各种应激反应基因，并参与杀虫剂抗性的发展。
结果
在这项研究中，鉴定并表征了蝗Locusta migratoria 的一个全长 CncC 序列。在 LmCncC 的 RNAi 沉默后，杀虫剂生物测定表明 LmCncC 在对溴氰菊酯和吡虫啉的敏感性中起重要作用。为了充分研究由 LmCncC 调节的下游基因，并进一步鉴定与溴氰菊酯和吡虫啉敏感性相关的 LmCncC 调节基因，构建了一个比较转录组。从 dsLmCncC 敲低个体中筛选出 35 个上调基因和 73 个下调基因。我们选择了 22 个 LmCncC 调节基因，并使用 RT-qPCR 验证了它们的基因表达水平。最后，选择了六个属于 CYP6 家族的 LmCYP450 基因作为候选解毒基因，并通过 RNAi、杀虫剂生物测定和代谢物鉴定进一步验证了 LmCYP6FD1 和 LmCYP6FE1 是杀虫剂的解毒基因。
结论
我们的数据表明，蝗 CncC 基因通过分别调节 LmCYP6FD1 和 LmCYP6FE1 与溴氰菊酯和吡虫啉的敏感性相关。
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