Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Pestic Biochem Physiol. 2023 Nov;196:105632. doi: 10.1016/j.pestbp.2023.105632. Epub 2023 Oct 12.
The cyclic AMP-responsive element-binding protein 3 (CREB3) members have unique regulatory roles in cellular lipid metabolism as transcription factors. Two CREB3 proteins in Nilaparvata lugens were identified and analyzed. In ovary, when silencing NlCREB3-2, triacylglycerol (TAG) content dramatically increased but glycerol and free fatty acid (FFA) significantly decreased, which implicated that NlCREB3-2 was involved in the lipase-related TAG metabolism. In N. lugens, five neutral lipases with complete features for TAG hydrolytic activity and high expression in ovary were focused. Among them, the expression levels of three neutral lipase genes were significantly down-regulated by NlCREB3-2 RNAi. The direct regulation of NlCREB3-2 towards the three neutral lipase genes was evidenced by the dual-luciferase reporter assay. After jointly silencing three neutral lipase genes, TAG and glycerol contents displayed similar changes as NlCREB3-2 RNAi. The study proved that NlCREB3-2 participated in TAG metabolism in ovary via the direct activation towards the ovary-specific neutral lipase genes.
环腺苷酸反应元件结合蛋白 3 (CREB3) 成员作为转录因子在细胞脂质代谢中具有独特的调节作用。在褐飞虱中鉴定并分析了两种 CREB3 蛋白。在卵巢中，沉默 NlCREB3-2 后，三酰甘油 (TAG) 含量显著增加，但甘油和游离脂肪酸 (FFA) 显著减少，这表明 NlCREB3-2 参与了脂肪酶相关的 TAG 代谢。在褐飞虱中，关注了五种具有完整 TAG 水解活性特征且在卵巢中高表达的中性脂肪酶。其中，三种中性脂肪酶基因的表达水平被 NlCREB3-2 RNAi 显著下调。双荧光素酶报告基因检测证实了 NlCREB3-2 对这三种中性脂肪酶基因的直接调控。共同沉默三种中性脂肪酶基因后，TAG 和甘油含量的变化与 NlCREB3-2 RNAi 相似。该研究证明，NlCREB3-2 通过直接激活卵巢特异性中性脂肪酶基因参与卵巢中的 TAG 代谢。
