Weldon School of Biomedical Engineering, Purdue University Flex Lab, West Lafayette, IN 47906, USA.
Krannert Cardiovascular Research Center, Division of Cardiovascular Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Mil Med. 2023 Nov 8;188(Suppl 6):474-479. doi: 10.1093/milmed/usad208.
Rodent models are often used in spinal cord injury investigations to measure physiological parameters but require rats to be restrained during data collection to prevent motion and stress-induced artifacts.
A 4-week acclimation protocol was developed to reduce sympathetic activity during experimentation to collect clean data. Physiological parameters were analyzed throughout the acclimation protocol using surface-based electrodes and an implanted sensor. The sensor was used to extract systolic blood pressure, skin nerve activity, and heart rate variability parameters.
Our protocol exposed a minimal increase in sympathetic activity during experimentation despite long periods of restraint. The data suggest that the acclimation protocol presented successfully minimized changes in physiological parameters because of prolonged restraint.
This is necessary to ensure that physiological recordings are not affected by undue stress because of the process of wearing the sensor. This is important when determining the effects of stress when studying dysautonomia after spinal cord injury, Parkinson's disease, and other neurological disorders.
啮齿动物模型常用于脊髓损伤研究中，以测量生理参数，但在数据收集过程中需要将大鼠固定，以防止运动和应激引起的伪影。
开发了一个 4 周的适应方案，以减少实验过程中的交感神经活动，从而收集清洁数据。使用基于表面的电极和植入式传感器在整个适应方案中分析生理参数。该传感器用于提取收缩压、皮肤神经活动和心率变异性参数。
尽管长时间固定，但我们的方案显示实验过程中交感神经活动仅略有增加。数据表明，适应方案成功地最小化了由于长时间固定导致的生理参数变化。
这对于确保生理记录不受传感器佩戴过程中过度应激的影响是必要的。当研究脊髓损伤、帕金森病和其他神经紊乱后自主神经功能紊乱时，确定应激的影响非常重要。
