一种快速 HPLC/UV 法测定细胞培养基中酮洛芬的含量。
A Fast HPLC/UV Method for Determination of Ketoprofen in Cellular Media.
机构信息
BIOCEV, First Faculty of Medicine, Charles University, 252 20, Vestec, Czech Republic.
Department of Analytical Chemistry, Faculty of Chemical Engineering, University of Chemistry and Technology, 166 28, Prague, Czech Republic.
出版信息
ChemistryOpen. 2024 Mar;13(3):e202300147. doi: 10.1002/open.202300147. Epub 2023 Nov 13.
A simple, sensitive and quick HPLC method was developed for the determination of ketoprofen in cell culture media (EMEM, DMEM, RPMI). Separation was performed using a gradient on the C18 column with a mobile phase of acetonitrile and miliQ water acidified by 0.1 % (v/v) formic acid. The method was validated for parameters including linearity, accuracy, precision, limit of quantitation and limit of detection, as well as robustness. The response was found linear over the range of 3-100 μg/mL as demonstrated by the acquired value of correlation coefficient R2=0.9997. The described method is applicable for determination of various pharmacokinetic aspects of ketoprofen in vitro.
建立了一种简单、灵敏、快速的 HPLC 方法，用于测定细胞培养基（EMEM、DMEM、RPMI）中的酮洛芬。分离采用 C18 柱梯度洗脱，流动相为乙腈和经 0.1%（v/v）甲酸酸化的 MilliQ 水。该方法对包括线性、准确性、精密度、定量限和检测限以及稳健性在内的参数进行了验证。结果表明，在 3-100μg/mL 范围内，相关系数 R2=0.9997，响应呈线性。所描述的方法适用于体外测定酮洛芬的各种药代动力学参数。
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