Centro de Investigaciones en Microbiología y Biotecnología-UR, Facultad de Ciencias Naturales, Universidad del Rosario, Bogota, Colombia.
Grupo de Investigación en Ciencias Animales, Facultad de Medicina Veterinaria y Zootecnia, Universidad Cooperativa de Colombia, Bucaramanga, Colombia.
Am J Trop Med Hyg. 2023 Nov 13;110(1):36-39. doi: 10.4269/ajtmh.23-0380. Print 2024 Jan 3.
Chagas disease (CD) is a parasitic infection caused by the parasite Trypanosoma cruzi. Reports of CD cases associated with oral transmission have increased, particularly in Colombia, Brazil, and Venezuela. In this investigation, parasitological, serological, and molecular tests were conducted on samples obtained from humans, mammal reservoirs, and hosts involved in the assessment of a suspected oral transmission outbreak in Cubara, Boyaca, Colombia. Seropositivity was observed in 60% (3 of 5) of index patients and 6.4% (5 of 78) of close contacts. Trypanosoma cruzi DNA was detected by quantitative polymerase chain reaction in 100% of index cases, 6.4% (5 of 78) of close contacts, 60% (6 of 10) of canines, and 100% (5 of 5) of opossums. In all index cases, the TcI lineage was identified, along with two cases of mixed infection (TcI/TcII-TcVI). Hemoculture revealed a flagellate presence in 80% of opossums, whereas all triatomine bugs tested negative. Our findings suggest a potential oral transmission route through contamination with opossum secretions.
恰加斯病（CD）是一种寄生虫感染，由寄生虫克氏锥虫引起。与口腔传播相关的 CD 病例报告有所增加，特别是在哥伦比亚、巴西和委内瑞拉。在本次调查中，对来自人类、哺乳动物储存宿主和宿主的样本进行了寄生虫学、血清学和分子检测，以评估哥伦比亚博亚卡省 Cubara 疑似口腔传播暴发。在 5 名指数病例中观察到 60%（3/5）的血清阳性，在 78 名密切接触者中观察到 6.4%（5/78）的血清阳性。定量聚合酶链反应检测到 100%（10/10）的犬和 100%（5/5）的负鼠的 TcI 谱系。在所有指数病例中均检测到 TcI 谱系，有两例混合感染（TcI/TcII-TcVI）。在 80%的负鼠的血培养中发现了鞭毛存在，而所有检测的三锥虫均为阴性。我们的研究结果表明，可能存在通过受负鼠分泌物污染的口腔传播途径。
