线粒体追踪剂：一个需要更好替代品的有用工具。

MitoTracker: A useful tool in need of better alternatives.

机构信息

Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.

Del Monte Institute for Neuroscience, Department of Neuroscience, University of Rochester, School of Medicine and Dentistry, Rochester 14642, USA.

出版信息

Eur J Cell Biol. 2023 Dec;102(4):151371. doi: 10.1016/j.ejcb.2023.151371. Epub 2023 Oct 31.

DOI:10.1016/j.ejcb.2023.151371

PMID:37956476

Abstract

The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design.

摘要

线粒体的荧光观察通常通过 MitoTracker 进行，MitoTracker 是一种亲脂性阳离子染料，可以被线粒体摄取。在本论坛中，我们重点介绍了 MitoTracker 可能出现的几个问题，包括对其他细胞器的染色。我们的目的是提供替代染料，并讨论它们的优缺点。我们还提供了替代 MitoTracker 的软件选项，以加快未来的实验设计。