Sánchez-Díez Silvia, Muñoz Xavier, Montalvo Tomás, Ojanguren Iñigo, Romero-Mesones Christian, Senar Juan Carlos, Peracho-Tobeña Victor, Cruz María-Jesús
Servicio de Neumología, Departamento de Medicina, Hospital Universitario Vall d'Hebron, Universidad Autónoma de Barcelona, Passeig Vall d'Hebron, 119, 08035, Barcelona, Spain.
CIBER Enfermedades Respiratorias (Ciberes), Madrid, Spain.
Allergy Asthma Clin Immunol. 2023 Nov 13;19(1):96. doi: 10.1186/s13223-023-00852-w.
Hypersensitivity pneumonitis (HP) is usually caused by the inhalation of avian and fungal proteins. The present study assesses a cohort of Urban Pest Surveillance and Control Service (UPSCS) workers with high exposure to avian and fungal antigens, in order to identify their degree of sensitization and the potential risk of developing HP.
Workers were divided according to their work activity into Nest pruners (Group 1) and Others (Group 2). All individuals underwent a medical interview, pulmonary function tests and the determination of specific IgG antibodies. Antigenic proteins of pigeon sera were analysed using two-dimensional immunoblotting. Proteins of interest were sequenced by liquid-chromatography-mass spectrometry (LC-MS).
101 workers were recruited (76 men, average age: 42 yrs); (Group 1 = 41, Group 2 = 60). Up to 30% of the study population exhibited increased levels of IgGs to pigeon, small parrot and parrot, and up to 60% showed high levels of Aspergillus and Penicillium IgGs. In Group 1, specific parakeet and Mucor IgGs were higher (p = 0.044 and 0.003 respectively) while DLCO/VA% were lower (p = 0.008) than in Group 2. Two-dimensional immunoblotting showed protein bands of 20-30 KDa recognized by HP patients but not by workers. LC-MS analysis identified Ig Lambda chain and Apolipoprotein A-I as candidate proteins for distinguishing HP patients from exposed workers.
Two pigeon proteins were identified that may play a role in the development of pathological differences between HP patients and exposed workers. DLCO/VA may have a predictive value in the development of HP disease.
过敏性肺炎（HP）通常由吸入禽类和真菌蛋白引起。本研究评估了一组高度暴露于禽类和真菌抗原的城市害虫监测与控制服务（UPSCS）工作人员，以确定他们的致敏程度和患HP的潜在风险。
根据工作活动将工作人员分为鸟巢修剪工（第1组）和其他人员（第2组）。所有个体均接受了医学访谈、肺功能测试和特异性IgG抗体测定。使用二维免疫印迹法分析鸽血清的抗原蛋白。通过液相色谱 - 质谱联用（LC - MS）对感兴趣的蛋白质进行测序。
招募了101名工作人员（76名男性，平均年龄：42岁）；（第1组 = 41人，第2组 = 60人）。高达30%的研究人群对鸽、小鹦鹉和鹦鹉的IgG水平升高，高达60%的人显示曲霉和青霉IgG水平较高。在第1组中，特定的长尾小鹦鹉和毛霉IgG较高（分别为p = 0.044和0.003），而与第2组相比，DLCO/VA%较低（p = 0.008）。二维免疫印迹显示20 - 30 kDa的蛋白条带可被HP患者识别，但工作人员不能识别。LC - MS分析确定Igλ链和载脂蛋白A - I为区分HP患者和暴露工作人员的候选蛋白。
鉴定出两种鸽蛋白，它们可能在HP患者和暴露工作人员之间病理差异的发展中起作用。DLCO/VA可能对HP疾病的发展具有预测价值。
