Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Int J Mol Sci. 2023 Nov 3;24(21):15928. doi: 10.3390/ijms242115928.
Grafting is widely used to enhance the phenotypic traits of tomatoes, alleviate biotic and abiotic stresses, and control soil-borne diseases of the scion in greenhouse production. There are many factors that affect the healing and acclimatization stages of seedlings after grafting. However, the role of light has rarely been studied. In this study, we compared the effects of artificial light and traditional shading (under shaded plastic-covered tunnels) on the recovery of grafted tomato seedlings. The results show that the grafted tomato seedlings recovered using artificial light had a higher healthy index, leaf chlorophyll content, shoot dry weight, and net photosynthetic rate (P) and water use efficiency (WUE) compared with grafted seedling recovered using the traditional shading method. Transcriptome analysis showed that the differentially expressed genes (DEGs) of grafted seedlings restored using artificial light were mainly enriched in the pathways corresponding to plant hormone signal transduction. In addition, we measured the endogenous hormone content of grafted tomato seedlings. The results show that the contents of salicylic acid (SA) and kinetin (Kin) were significantly increased, and the contents of indoleacetic acid (IAA) and jasmonic acid (JA) were decreased in artificial-light-restored grafted tomato seedlings compared with those under shading treatments. Therefore, we suggest that artificial light affects the morphogenesis and photosynthetic efficiency of grafted tomato seedlings, and it can improve the performance of tomato seedlings during grafting recovery by regulating endogenous hormone levels.
嫁接广泛用于增强番茄的表型特征、缓解生物和非生物胁迫，并控制温室生产中接穗的土传病害。有许多因素会影响嫁接后幼苗的愈合和适应阶段。然而，光照的作用很少被研究。在这项研究中，我们比较了人工光和传统遮荫（在覆盖有遮阳塑料的隧道下）对嫁接番茄幼苗恢复的影响。结果表明，与传统遮荫法恢复的嫁接番茄幼苗相比，人工光恢复的嫁接番茄幼苗具有更高的健康指数、叶片叶绿素含量、茎干重以及净光合速率（P）和水分利用效率（WUE）。转录组分析表明，人工光恢复的嫁接幼苗的差异表达基因（DEGs）主要富集在与植物激素信号转导相对应的途径中。此外，我们还测量了嫁接番茄幼苗的内源激素含量。结果表明，与遮荫处理相比，人工光恢复的嫁接番茄幼苗中水杨酸（SA）和激动素（Kin）的含量显著增加，吲哚乙酸（IAA）和茉莉酸（JA）的含量降低。因此，我们认为人工光会影响嫁接番茄幼苗的形态发生和光合效率，并且可以通过调节内源激素水平来改善嫁接恢复期间番茄幼苗的性能。
