College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.
Sci Rep. 2019 Dec 27;9(1):20240. doi: 10.1038/s41598-019-56563-z.
Tomato is an important vegetable crop grown worldwide. Grafting is an agricultural technique that is used to improve growth, yield, and resistance to diverse stresses in tomato production. Here, we examined the differences between the scion of heterografted ('Provence'/'Haomei') and homografted ('Provence'/'Provence') tomato seedlings. We observed anatomical changes during the graft-union healing process in heterografted and homografted tomato seedlings and conducted transcriptome analyses of the 'Provence' scion from both graft combinations. With the development of calli from both graft partners, the isolation layer became thinner at 16 d after grafting (DAG). Compared with that of homografts, the healing in heterografts was slightly delayed, but the graft union had completely healed at 21 DAG. In total, 858 significantly differentially expressed genes were detected between the transcriptomes of heterografts and homografts at 16 DAG. Functional pathways identified by GO and KEGG enrichment analyses were associated with primary and secondary metabolism, hormone signalling, transcription factor regulation, transport, and responses to stimuli. Many differentially expressed genes were involved in pathways associated with mitogen-activated protein kinase signalling, plant hormone signalling, and oxidative stress. A number of transcription factors were up-regulated in the scion of heterografted seedlings. The results provide a valuable resource for the elucidation of the molecular mechanisms, and candidate genes for functional analyses, of heterograft and homograft systems.
番茄是全球广泛种植的重要蔬菜作物。嫁接是一种农业技术，用于提高番茄生产中的生长、产量和对多种胁迫的抗性。在这里，我们研究了异接（'Provence'/ 'Haomei'）和同接（'Provence'/'Provence'）番茄幼苗接穗之间的差异。我们观察了异接和同接番茄幼苗嫁接愈合过程中的解剖学变化，并对来自这两种嫁接组合的'Provence'接穗进行了转录组分析。随着来自两个嫁接伙伴的愈伤组织的发育，在嫁接后 16 天（DAG）时隔离层变得更薄。与同接相比，异接的愈合稍有延迟，但在 21 DAG 时嫁接愈合完全。在 16 DAG 时，异接和同接的转录组之间共检测到 858 个显著差异表达基因。GO 和 KEGG 富集分析确定的功能途径与初级和次级代谢、激素信号转导、转录因子调控、运输以及对刺激的反应有关。许多差异表达基因参与与丝裂原激活蛋白激酶信号转导、植物激素信号转导和氧化应激相关的途径。许多转录因子在异接幼苗的接穗中上调。研究结果为阐明异接和同接系统的分子机制和候选基因功能分析提供了有价值的资源。
