Yu Lishu, Bu Lijun, Li Dandan, Zhu Kaili, Zhang Yongxue, Wu Shaofang, Chang Liying, Ding Xiaotao, Jiang Yuping
College of Ecological Technology and Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
Shanghai Key Laboratory of Protected Horticultural Technology, Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Plants (Basel). 2024 Dec 14;13(24):3495. doi: 10.3390/plants13243495.
To investigate the effects of different light qualities on the growth, photosynthesis, transcriptome, and metabolome of mint, three treatments were designed: (1) 7R3B (70% red light and 30% blue light, CK); (2) 7R3B+ far-red light (FR); (3) 7R3B+ ultraviolet light A (UVA). The results showed that supplemental FR significantly promoted the growth and photosynthesis of mint, as evidenced by the increase in plant height, plant width, biomass, effective quantum yield of PSII photochemistry (F'/F'), maximal quantum yield of PSII (F/F), and performance index (PI). UVA and CK exhibited minimal differences. Transcriptomic and metabolomic analysis indicated that a total of 788 differentially expressed genes (DEGs) and 2291 differential accumulated metabolites (DAMs) were identified under FR treatment, mainly related to plant hormone signal transduction, phenylpropanoid biosynthesis, and flavonoid biosynthesis. FR also promoted the accumulation of phenylalanine, sinapyl alcohol, methylchavicol, and anethole in the phenylpropanoid biosynthesis pathway, and increased the levels of luteolin and leucocyanidin in the flavonoid biosynthesis pathway, which may perhaps be applied in practical production to promote the natural antibacterial and antioxidant properties of mint. An appropriate increase in FR radiation might alter transcript reprogramming and redirect metabolic flux in mint, subsequently regulating its growth and secondary metabolism. Our study uncovered the regulation of FR and UVA treatments on mint in terms of growth, physiology, transcriptome, and metabolome, providing reference for the cultivation of mint and other horticultural plants.
为了研究不同光质对薄荷生长、光合作用、转录组和代谢组的影响，设计了三种处理：（1）7R3B（70%红光和30%蓝光，对照）；（2）7R3B+远红光（FR）；（3）7R3B+紫外线A（UVA）。结果表明，补充FR显著促进了薄荷的生长和光合作用，植物高度、株幅、生物量、PSII光化学有效量子产率（F'/F'）、PSII最大量子产率（F/F）和性能指数（PI）的增加证明了这一点。UVA和对照表现出最小的差异。转录组和代谢组分析表明，在FR处理下共鉴定出788个差异表达基因（DEG）和2291个差异积累代谢物（DAM），主要与植物激素信号转导、苯丙烷生物合成和类黄酮生物合成有关。FR还促进了苯丙烷生物合成途径中苯丙氨酸、芥子醇、甲基丁香酚和茴香脑的积累，并增加了类黄酮生物合成途径中木犀草素和无色花青素的水平，这可能在实际生产中用于促进薄荷的天然抗菌和抗氧化特性。适当增加FR辐射可能会改变薄荷的转录重编程并重新引导代谢通量，随后调节其生长和次生代谢。我们的研究揭示了FR和UVA处理对薄荷在生长、生理、转录组和代谢组方面的调控，为薄荷和其他园艺植物的栽培提供了参考。
