Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 145, XingDa Rd., Taichung, 40227, Taiwan, ROC.
Department of Energy, Environmental and Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
Int J Syst Evol Microbiol. 2023 Nov;73(11). doi: 10.1099/ijsem.0.006146.
A polyphasic taxonomic approach was used to characterize a novel bacterium, designated strain CC-YST667, isolated from poultry manure sampled in Taiwan. The cells were observed to be aerobic, motile and non-spore-forming rods, displaying positive reactions for oxidase. Optimal growth of CC-YST667 was observed at 25 °C, pH 8.0 and with 1 % (w/v) NaCl. The polar lipid profile consisted of phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and multiple unidentified polar lipids. The major polyamine was spermidine. The major cellular fatty acids (>5 %) included C, Ccyclo, Ccyclo ω8 and C 3OH/iso-C I. On the basis of the results of analysis of 16S rRNA gene sequences, this isolate showed the closest phylogenetic relationship with ' (with 98.2 % similarity) and (with 97.3 % similarity) of the family . The draft genome, (3.3 Mb) with a DNA G+C content of 57.2 mol%, harboured various genes involved in the biodegradation of aromatic hydrocarbons. CC-YST667 shared highest orthologous average nucleotide identity (OrthoANI) with the type strains of species of of the genera (72.4‒77.9 %, =2), (72.8‒73.0 %, =2) and (71.7‒73.0 %, =5). On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic traits together with the results of comparative 16S rRNA gene sequencing, OrthoANI, digital DNA-DNA hybridization (DDH) and the phylogenomic placement, strain CC-YST667 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CC-YST667 (=BCRC 81321 =JCM 34761).
采用多相分类方法对一株从台湾禽粪中分离到的新型细菌进行了研究，命名为 CC-YST667 菌株。观察到细胞为需氧、能动、非孢子形成的杆状，氧化酶呈阳性反应。CC-YST667 的最佳生长温度为 25°C，pH 值为 8.0，NaCl 浓度为 1%（w/v）。极性脂谱由磷脂单甲乙醇胺、磷脂酰甘油、双磷脂酰甘油、磷脂乙醇胺和多种未鉴定的极性脂组成。主要聚胺为亚精胺。主要细胞脂肪酸（>5%）包括 C、Ccyclo、Ccyclo ω8 和 C3OH/iso-C I。基于 16S rRNA 基因序列分析的结果，该分离株与家族的 （相似度为 98.2%）和 （相似度为 97.3%）最为密切相关。基因组草图（3.3 Mb），GC 含量为 57.2 mol%，包含各种参与芳香烃生物降解的基因。CC-YST667 与种的模式菌株的最高同源平均核苷酸同一性（OrthoANI）与属的 （72.4-77.9%，=2）、 （72.8-73.0%，=2）和 （71.7-73.0%，=5）最为接近。基于其独特的系统发育、表型和化学分类特征以及比较 16S rRNA 基因测序、OrthoANI、数字 DNA-DNA 杂交（DDH）和系统发育定位的结果，CC-YST667 菌株被认为代表了一个新的属，提议将其命名为 sp. nov.。模式菌株为 CC-YST667（=BCRC 81321 =JCM 34761）。
