Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung City, Taiwan, ROC.
Yenepoya Research Centre, Yenepoya Deemed to be University, Mangalore, Karnataka, India.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005111.
A polyphasic taxonomic approach was used to characterize a Gram-stain-negative bacterium, designated strain CC-CFT640, isolated from vineyard soil sampled in Taiwan. Cells of strain CC-CFT640 were aerobic, non-motile, nitrate-reducing rods. Test results were positive for catalase, oxidase and proteinase activities. Optimal growth occurred at 30 °С and pH 7. Strain CC-CFT640 showed highest 16S rRNA gene sequence similarity to members of the genus (90.0 %, =1) followed by (89.4-90.0 %, =2), (88.8-89.8 %, =5) and (89.2-89.4 %, =2), and formed a distinct phyletic lineage distantly associated with the clade that predominately accommodated species. The DNA G+C composition of the genome (2.1 Mb) was 67.9 mol%. Genes involved in the reduction of nitrate to nitrite, nitric oxide and nitrous oxide were found. In addition, genes encoding dissimilatory nitrate reduction to ammonia, ammonium transport and ammonium assimilation were also detected. Average nucleotide identity values were 73.3 % (=1), 74.0-74.6 % (=2), 67.5-68.3 % (=2) when compared within the type strains of the genera , and , respectively. The dominant cellular fatty acids (>5 %) included C, iso-C 10, C cyclo 8, C 2-OH and C 7/C 6. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified aminolipids, three unidentified phospholipids and an unidentified aminophospholipid. The major respiratory quinone was ubiquinone 10 and the major polyamine was spermidine. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequencing, digital DNA-DNA hybridization, average nucleotide identity and phylogenomic placement, strain CC-CFT640 is considered to represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain is CC-CFT640 (=BCRC 81219=JCM 33507).
采用多相分类法对一株革兰氏阴性细菌进行了表征，该菌命名为 CC-CFT640 菌株，分离自台湾葡萄园土壤。CC-CFT640 菌株为需氧、非运动、硝酸盐还原杆状菌。试验结果表明该菌具有触酶、氧化酶和蛋白酶活性。最适生长温度为 30°C，pH 值为 7。CC-CFT640 菌株与属内的成员具有最高的 16S rRNA 基因序列相似性（90.0%，=1），其次是 （89.4-90.0%，=2）、 （88.8-89.8%，=5）和 （89.2-89.4%，=2），并与主要容纳 种的分支形成一个独特的系统发育谱系。基因组（2.1 Mb）的 DNA G+C 含量为 67.9 mol%。发现了参与将硝酸盐还原为亚硝酸盐、一氧化氮和氧化亚氮的基因。此外，还检测到了异化硝酸盐还原为氨、铵转运和铵同化的基因。与属内的模式菌株相比，平均核苷酸同一性值分别为 73.3%（=1）、74.0-74.6%（=2）、67.5-68.3%（=2）。细胞脂肪酸的主要成分（>5%）包括 C、iso-C 10、C 环 8、C 2-OH 和 C 7/C 6。极性脂谱由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、三种未鉴定的氨基脂、三种未鉴定的磷脂和一种未鉴定的氨基磷脂组成。主要呼吸醌是泛醌 10，主要多胺是亚精胺。基于其独特的系统发育、表型和化学分类特征以及比较 16S rRNA 基因测序、数字 DNA-DNA 杂交、平均核苷酸同一性和系统基因组位置的结果，CC-CFT640 菌株被认为代表了一个新的属和种，该属和种被提议命名为 属，新种，新种。模式菌株为 CC-CFT640（=BCRC 81219=JCM 33507）。
