Center for Molecular Spectroscopy and Dynamics, Institute for Basic Science, Seoul, Korea.
Department of Physics, Korea University, Seoul, Korea.
Nat Commun. 2023 Nov 14;14(1):7160. doi: 10.1038/s41467-023-42347-7.
A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood. Here, we used interferometric scattering microscopy to track unlabeled cargos in directional motion in a massively parallel fashion. Our label-free, cargo-tracing method revealed not only the dynamics of cargo transportation but also the fine architecture of the actively used cytoskeletal highways and the long-term evolution of the associated traffic at sub-diffraction resolution. Cargos frequently run into a blocked road or experience a traffic jam. Still, they have effective strategies to circumvent those problems: opting for an alternative mode of transport and moving together in tandem or migrating collectively. All taken together, a cell is an incredibly complex and busy space where the principle and practice of transportation intriguingly parallel those of our macroscopic world.
真核细胞是一个微观世界，其中有效的物质运输是必不可少的。然而，细胞如何在拥挤的环境中有效地输送细胞货物仍知之甚少。在这里，我们使用干涉散射显微镜以大规模并行的方式跟踪定向运动中的未标记货物。我们的无标记货物追踪方法不仅揭示了货物运输的动力学，还揭示了活跃使用的细胞骨架高速公路的精细结构，以及亚衍射分辨率下相关交通的长期演变。货物经常会遇到阻塞的道路或交通堵塞。尽管如此，它们还是有有效的策略来规避这些问题：选择另一种运输方式，或者一起并驾齐驱，或者集体迁移。总的来说，细胞是一个令人难以置信的复杂和繁忙的空间，其中运输的原则和实践与我们的宏观世界惊人地相似。
